Comparison of two chromogenic media for the detection of vancomycin-resistant enterococcal carriage by nursing home residents

Theodore Gouliouris*, Beth Blane, Hayley J. Brodrick, Kathy E. Raven, Kirsty E. Ambridge, Angela D. Kidney, Nazreen F. Hadjirin, M. Estée Török, Direk Limmathurotsakul, Sharon Peacock

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    6 Citations (Scopus)

    Abstract

    We compared ChromID VRE and Brilliance VRE media for the detection of vancomycin-resistant enterococci (VRE). Using a panel of 28 enterococcal isolates, 10 vanA Enterococcus faecium and three vanA Enterococcus faecalis isolates grew as per manufacturers’ instructions whilst growth of two vanC and eight vancomycin-susceptible enterococci was inhibited on both media. Important differences were noted in the selectivity and chromogenic properties of the two media for vanA Enterococcus raffinosus and vanB E. faecium. The two media were further evaluated using 295 stool samples from nursing home residents, 34 of which grew VRE (11.5%). ChromID and Brilliance had comparable sensitivity, which was increased markedly by prolonging incubation to 48 hours (from 29% to 82%, and from 41% to 85%, respectively) and by a pre-enrichment step (to 97% and 100%, respectively). Brilliance VRE agar had higher selectivity at 48 hours, and after pre-enrichment.

    Original languageEnglish
    Pages (from-to)409-412
    Number of pages4
    JournalDiagnostic Microbiology and Infectious Disease
    Volume85
    Issue number4
    DOIs
    Publication statusPublished - 1 Aug 2016

    Bibliographical note

    Publisher Copyright:
    © 2016 The Authors

    Keywords

    • Detection
    • Enterococcus faecium
    • Selectivity
    • Sensitivity
    • VRE
    • Vancomycin-resistant enterococci

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