TY - JOUR
T1 - Comparison of two chromogenic media for the detection of vancomycin-resistant enterococcal carriage by nursing home residents
AU - Gouliouris, Theodore
AU - Blane, Beth
AU - Brodrick, Hayley J.
AU - Raven, Kathy E.
AU - Ambridge, Kirsty E.
AU - Kidney, Angela D.
AU - Hadjirin, Nazreen F.
AU - Török, M. Estée
AU - Limmathurotsakul, Direk
AU - Peacock, Sharon
N1 - Publisher Copyright:
© 2016 The Authors
PY - 2016/8/1
Y1 - 2016/8/1
N2 - We compared ChromID VRE and Brilliance VRE media for the detection of vancomycin-resistant enterococci (VRE). Using a panel of 28 enterococcal isolates, 10 vanA Enterococcus faecium and three vanA Enterococcus faecalis isolates grew as per manufacturers’ instructions whilst growth of two vanC and eight vancomycin-susceptible enterococci was inhibited on both media. Important differences were noted in the selectivity and chromogenic properties of the two media for vanA Enterococcus raffinosus and vanB E. faecium. The two media were further evaluated using 295 stool samples from nursing home residents, 34 of which grew VRE (11.5%). ChromID and Brilliance had comparable sensitivity, which was increased markedly by prolonging incubation to 48 hours (from 29% to 82%, and from 41% to 85%, respectively) and by a pre-enrichment step (to 97% and 100%, respectively). Brilliance VRE agar had higher selectivity at 48 hours, and after pre-enrichment.
AB - We compared ChromID VRE and Brilliance VRE media for the detection of vancomycin-resistant enterococci (VRE). Using a panel of 28 enterococcal isolates, 10 vanA Enterococcus faecium and three vanA Enterococcus faecalis isolates grew as per manufacturers’ instructions whilst growth of two vanC and eight vancomycin-susceptible enterococci was inhibited on both media. Important differences were noted in the selectivity and chromogenic properties of the two media for vanA Enterococcus raffinosus and vanB E. faecium. The two media were further evaluated using 295 stool samples from nursing home residents, 34 of which grew VRE (11.5%). ChromID and Brilliance had comparable sensitivity, which was increased markedly by prolonging incubation to 48 hours (from 29% to 82%, and from 41% to 85%, respectively) and by a pre-enrichment step (to 97% and 100%, respectively). Brilliance VRE agar had higher selectivity at 48 hours, and after pre-enrichment.
KW - Detection
KW - Enterococcus faecium
KW - Selectivity
KW - Sensitivity
KW - VRE
KW - Vancomycin-resistant enterococci
UR - http://www.scopus.com/inward/record.url?scp=84989940562&partnerID=8YFLogxK
U2 - 10.1016/j.diagmicrobio.2016.04.026
DO - 10.1016/j.diagmicrobio.2016.04.026
M3 - Article
C2 - 27312688
AN - SCOPUS:84989940562
SN - 0732-8893
VL - 85
SP - 409
EP - 412
JO - Diagnostic Microbiology and Infectious Disease
JF - Diagnostic Microbiology and Infectious Disease
IS - 4
ER -