Comparison of ThinPrep and SurePath liquid-based cytology and subsequent human papillomavirus DNA testing in China

Fang Hui Zhao, Shang Ying Hu, Jessica J. Bian, Bin Liu, Roger B. Peck, Yan Ping Bao, Qin Jing Pan, Lucien Frappart, John Sellors, You Lin Qiao*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

37 Citations (Scopus)

Abstract

BACKGROUND: Liquid-based cytology (LBC) has been compared with conventional cytology in numerous studies. In the current study of 2 LBC systems, the accuracy, rates of unsatisfactory cytology, and sufficiency of residual LBC specimens for Hybrid Capture 2 (HC2) HPV DNA testing were compared. METHODS: Eligible women ages 30 to 49 years were recruited for this cross-sectional population-based study in rural China.Women were assessed by visual inspection with acetic acid (VIA), LBC, and high-risk HPV HC2 DNA assay. Cervical specimens were preserved according to SurePath or ThinPrep protocols. LBC results were manually read. HC2 testing was performed on specimens with sufficient residual volume. Colposcopies and biopsies were performed on women who were VIA positive at the time of initial screening. Women with abnormal LBC or HC2 test results were called back for colposcopies and 4-quadrant cervical biopsies. RESULTS: Of 2005 eligible women, 972 were tested by SurePath and 1033 by ThinPrep. Compared with SurePath samples, ThinPrep samples had higher rates of unsatisfactory cytology (0.2% for SurePath and 1.5% for ThinPrep) and insufficient residual volume for HC2 (0.0% for SurePath and 18.2% for ThinPrep). SurePath samples yielded higher sensitivities and similar specificities for LBC and HC2 testing of residual specimens, but these differences were not determined to be significant by area-under-the-curve analysis (LBC performance: 0.89 for SurePath and 0.85 for Thin-Prep; HC2 performance: 0.91 for SurePath and 0.89 for ThinPrep). CONCLUSIONS: Both methods yielded similar validity in detecting significant cervical lesions. However, SurePath samples yielded higher rates of satisfactory LBC slides and sufficient residual volume for HC2.

Original languageEnglish
Pages (from-to)387-394
Number of pages8
JournalCancer Cytopathology
Volume119
Issue number6
DOIs
Publication statusPublished - 25 Dec 2011
Externally publishedYes

Keywords

  • Cervical cancer
  • Cytology
  • DNA testing
  • Human papillomavirus (HPV)
  • Screening

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