Comparison of single radial immunodiffusion, SDS-PAGE and HPLC potency assays for inactivated influenza vaccines shows differences in ability to predict immunogenicity of haemagglutinin antigen

Othmar G. Engelhardt*, Chantal Edge, Una Dunleavy, Kate Guilfoyle, Ruth Harvey, Diane Major, Robert Newman, Rebecca Penn, Sarah Skeldon, Claire Storey, Jun Wheeler, John Wood, Philip Minor

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

The current gold-standard potency test for inactivated influenza vaccines is the single radial immunodiffusion (SRD) assay. A number of alternative potency tests for inactivated influenza vaccines have been proposed in recent years. Evaluation of these new potency tests commonly involves comparison with SRD, in order to ascertain that the new method obtains values that correlate with those measured by the standard potency test. Here, we extended comparison of two methods, reverse-phase HPLC and SDS-PAGE, with SRD by assessing the methods’ capacity to detect loss of potency induced by various deliberate treatments of vaccine samples. We demonstrate that neither of these methods detected the loss of potency observed by SRD; importantly, neither SDS-PAGE nor reverse-phase HPLC reflected results from mouse experiments that showed decreased immunogenicity and protection in vivo. These results emphasise the importance of assessing the stability-indicating nature, ie the ability to measure loss of vaccine potency, of any potential new potency assay.

Original languageEnglish
Pages (from-to)4339-4345
Number of pages7
JournalVaccine
Volume36
Issue number29
DOIs
Publication statusPublished - 5 Jul 2018
Externally publishedYes

Bibliographical note

Funding Information:
We acknowledge the help of NIBSC's Biological Services Division in the animal experiments conducted during this study and Peter Rigsby for statistical analysis.

Publisher Copyright:
© 2018

Keywords

  • Forced degradation
  • Influenza vaccine
  • Potency testing
  • SRD
  • SRID

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