Comparison of plaque reduction neutralisation test (PRNT) and measles virus-specific IgG ELISA for assessing immunogenicity of measles vaccination

B. J. Cohen*, D. Doblas, Nicholas Andrews

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

56 Citations (Scopus)

Abstract

Background: Plaque reduction neutralisation test (PRNT) is a gold standard assay for measles antibodies. PRNT, however, is time-consuming and more difficult to standardise than ELISA. Methods: PRNT results were obtained on pre- and post-vaccination sera from 501 measles-vaccinated infants. From this cohort a sub-set of 210 infants were selected for testing by ELISA, which was performed either manually or by an automated processor. Results were corrected for sampling proportion before comparing the assays. Results: Seroconversion was detected by PRNT in 454 infants of whom 324 were estimated to have seroconverted by manual ELISA and 191 by automated ELISA It is recommended that ELISA negative post-vaccination samples are re-tested by PRNT to detect all seroconversions.

Original languageEnglish
Pages (from-to)6392-6397
Number of pages6
JournalVaccine
Volume26
Issue number50
DOIs
Publication statusPublished - 25 Nov 2008

Bibliographical note

Funding Information:
The authors alone are responsible for views expressed in this manuscript and they do not necessarily represent the decisions, policy or views of the World Health Organization. World Health Organization Initiative for Vaccine Research (WHO/IVR) co-ordinates the Measles Aerosol Project (MAP). The Centre for Disease Control and Prevention (CDC), USA and, the American Red Cross (ARC) are partners in MAP, which has received financial support from the Bill and Melinda Gates Foundation. We thank Dr Ana Maria Henao Restrepo, focal point for MAP at WHO/IVR, for encouraging this work. We also thank Helmut Peters and Andrea Brock of Siemens Medical Solutions (formerly Dade Behring) for advice on performing measles IgG ELISA using BEP III processor.

Keywords

  • ELISA
  • Measles
  • Plaque reduction neutralisation test

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