Comparison of large-scale mammalian cell culture systems with egg culture for the production of influenza virus A vaccine strains

Julia Tree; Catherine Richardson; Anthony R. Fooks; J. Christopher Clegg; Denis Looby

doi:10.1016/S0264-410X(01)00053-6

Comparison of large-scale mammalian cell culture systems with egg culture for the production of influenza virus A vaccine strains

Julia Tree^*, Catherine Richardson, Anthony R. Fooks, J. Christopher Clegg, Denis Looby

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

134 Citations (Scopus)

Abstract

Different types of microcarriers were assessed for the large-scale culture of influenza virus in the Madin-Darby canine kidney (MDCK) cells. Both porous and solid carriers were examined. A higher titre of influenza A/PR8/34 virus was recovered from cultures using solid (1.3 × 10⁹ PFU per ml) rather than porous carriers (4.0 × 10⁸ PFU per ml). High titres of virus (1.0 × 10⁹ PFU per ml) were also obtained from roller bottle cultures of MDCK cells and the traditional culture technique using embryonated hens' eggs (3.9 × 10⁹ PFU per ml). We found that solid carriers composed of dextran with a positive charge are the most suitable carriers for the large-scale growth of influenza A virus in MDCK cells using serum-free media. Crown

Original language	English
Pages (from-to)	3444-3450
Number of pages	7
Journal	Vaccine
Volume	19
Issue number	25-26
DOIs	https://doi.org/10.1016/S0264-410X(01)00053-6
Publication status	Published - 14 May 2001

Bibliographical note

Funding Information:
This work was funded by the Department of Health, UK. Egg-adapted human influenza reassortant, prototype A/PR8/34 was obtained from Dr J Wood, NIBSC for which we are very grateful. We are grateful for the technical assistance of Mrs Hilary House. We would also like to thank Dr Ian Brown (VLA, Weybridge, Surrey) and Dr Maria Zambon (PHLS, Colindale, London) for critically reading this manuscript.

Keywords

Influenza
MDCK
Mammalian cell-culture
Microcarrier
Vaccine

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/S0264-410X(01)00053-6

Cite this

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title = "Comparison of large-scale mammalian cell culture systems with egg culture for the production of influenza virus A vaccine strains",

abstract = "Different types of microcarriers were assessed for the large-scale culture of influenza virus in the Madin-Darby canine kidney (MDCK) cells. Both porous and solid carriers were examined. A higher titre of influenza A/PR8/34 virus was recovered from cultures using solid (1.3 × 109 PFU per ml) rather than porous carriers (4.0 × 108 PFU per ml). High titres of virus (1.0 × 109 PFU per ml) were also obtained from roller bottle cultures of MDCK cells and the traditional culture technique using embryonated hens' eggs (3.9 × 109 PFU per ml). We found that solid carriers composed of dextran with a positive charge are the most suitable carriers for the large-scale growth of influenza A virus in MDCK cells using serum-free media. Crown",

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author = "Julia Tree and Catherine Richardson and Fooks, {Anthony R.} and Clegg, {J. Christopher} and Denis Looby",

note = "Funding Information: This work was funded by the Department of Health, UK. Egg-adapted human influenza reassortant, prototype A/PR8/34 was obtained from Dr J Wood, NIBSC for which we are very grateful. We are grateful for the technical assistance of Mrs Hilary House. We would also like to thank Dr Ian Brown (VLA, Weybridge, Surrey) and Dr Maria Zambon (PHLS, Colindale, London) for critically reading this manuscript.",

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AU - Richardson, Catherine

AU - Fooks, Anthony R.

AU - Clegg, J. Christopher

AU - Looby, Denis

N1 - Funding Information: This work was funded by the Department of Health, UK. Egg-adapted human influenza reassortant, prototype A/PR8/34 was obtained from Dr J Wood, NIBSC for which we are very grateful. We are grateful for the technical assistance of Mrs Hilary House. We would also like to thank Dr Ian Brown (VLA, Weybridge, Surrey) and Dr Maria Zambon (PHLS, Colindale, London) for critically reading this manuscript.

PY - 2001/5/14

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N2 - Different types of microcarriers were assessed for the large-scale culture of influenza virus in the Madin-Darby canine kidney (MDCK) cells. Both porous and solid carriers were examined. A higher titre of influenza A/PR8/34 virus was recovered from cultures using solid (1.3 × 109 PFU per ml) rather than porous carriers (4.0 × 108 PFU per ml). High titres of virus (1.0 × 109 PFU per ml) were also obtained from roller bottle cultures of MDCK cells and the traditional culture technique using embryonated hens' eggs (3.9 × 109 PFU per ml). We found that solid carriers composed of dextran with a positive charge are the most suitable carriers for the large-scale growth of influenza A virus in MDCK cells using serum-free media. Crown

AB - Different types of microcarriers were assessed for the large-scale culture of influenza virus in the Madin-Darby canine kidney (MDCK) cells. Both porous and solid carriers were examined. A higher titre of influenza A/PR8/34 virus was recovered from cultures using solid (1.3 × 109 PFU per ml) rather than porous carriers (4.0 × 108 PFU per ml). High titres of virus (1.0 × 109 PFU per ml) were also obtained from roller bottle cultures of MDCK cells and the traditional culture technique using embryonated hens' eggs (3.9 × 109 PFU per ml). We found that solid carriers composed of dextran with a positive charge are the most suitable carriers for the large-scale growth of influenza A virus in MDCK cells using serum-free media. Crown

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KW - MDCK

KW - Mammalian cell-culture

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JF - Vaccine

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ER -

Comparison of large-scale mammalian cell culture systems with egg culture for the production of influenza virus A vaccine strains

Abstract

Bibliographical note

Keywords

UN SDGs

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