TY - JOUR
T1 - Comparison of HPV genotypes and viral load between different sites of genital tract
T2 - The significance for cervical cancer screening
AU - Zhang, Shao Kai
AU - Ci, Pu Wa
AU - Velicer, Christine
AU - Kang, Le Ni
AU - Liu, Bin
AU - Cui, Jian Feng
AU - Chen, Feng
AU - Zhang, Xun
AU - Chang, Irene J.
AU - Roberts, Christine C.
AU - Smith, Jennifer S.
AU - Chen, Wen
AU - Qiao, You Lin
PY - 2014/4
Y1 - 2014/4
N2 - Objectives: To compare the consistency of HPV genotype and viral loads among different sites within the female genital tract, and to correlate these with clinical outcomes. Methods: 2646 previously unscreened rural women were enrolled in this population-based, cross-sectional study between May 2006 and April 2007. Physician-collected samples from lower vagina, upper vagina, cervix, and one self-collected sample were taken from each woman. Viral load was assessed by HC2 using the relative light unit/cutoff ratio (RLU/CO), and HPV genotyping was tested by Linear Array. Results: The low risk HPV positive rate was highest in lower vagina samples and lowest in cervix samples. Overall kappa values of high risk HPV types between various anatomic sampling sites showed substantial or almost perfect agreement among women with normal pathology, CIN1, and CIN2+. In the CIN2+ population, high risk HPV viral load for cervix samples (557.25. RLU/CO) were much higher than upper vagina samples (96.43. RLU/CO, P<. 0.001), lower vagina samples (36.51. RLU/CO, P<. 0.001), and self-collected (206.83. RLU/CO, P= 0.003) samples. Conclusions: Although the distribution of high risk HPV genotypes was fairly equivalent across different genital sites, particularly for CIN2+ lesions, viral loads were largely variable. The findings may affect the cervical cancer screening methods using self-collected samples, particularly in resource-challenged areas.
AB - Objectives: To compare the consistency of HPV genotype and viral loads among different sites within the female genital tract, and to correlate these with clinical outcomes. Methods: 2646 previously unscreened rural women were enrolled in this population-based, cross-sectional study between May 2006 and April 2007. Physician-collected samples from lower vagina, upper vagina, cervix, and one self-collected sample were taken from each woman. Viral load was assessed by HC2 using the relative light unit/cutoff ratio (RLU/CO), and HPV genotyping was tested by Linear Array. Results: The low risk HPV positive rate was highest in lower vagina samples and lowest in cervix samples. Overall kappa values of high risk HPV types between various anatomic sampling sites showed substantial or almost perfect agreement among women with normal pathology, CIN1, and CIN2+. In the CIN2+ population, high risk HPV viral load for cervix samples (557.25. RLU/CO) were much higher than upper vagina samples (96.43. RLU/CO, P<. 0.001), lower vagina samples (36.51. RLU/CO, P<. 0.001), and self-collected (206.83. RLU/CO, P= 0.003) samples. Conclusions: Although the distribution of high risk HPV genotypes was fairly equivalent across different genital sites, particularly for CIN2+ lesions, viral loads were largely variable. The findings may affect the cervical cancer screening methods using self-collected samples, particularly in resource-challenged areas.
KW - Genital tract
KW - Genotype
KW - Human papillomavirus
KW - Intraepithelial neoplasia
KW - Viral load
UR - http://www.scopus.com/inward/record.url?scp=84898022395&partnerID=8YFLogxK
U2 - 10.1016/j.canep.2014.01.004
DO - 10.1016/j.canep.2014.01.004
M3 - Article
C2 - 24522235
AN - SCOPUS:84898022395
SN - 1877-7821
VL - 38
SP - 168
EP - 173
JO - Cancer Epidemiology
JF - Cancer Epidemiology
IS - 2
ER -
