Comparison of four-colour IS6110-fAFLP with the classic IS6110-RFLP on the ability to detect recent transmission in the city of Barcelona, Spain

Sònia Borrell; Nicola Thorne; Montserrat Español; Chloe Mortimer; Àngels Orcau; Pere Coll; Saheer Gharbia; Julian González-Martín; Catherine Arnold

doi:10.1016/j.tube.2009.03.003

Comparison of four-colour IS6110-fAFLP with the classic IS6110-RFLP on the ability to detect recent transmission in the city of Barcelona, Spain

Sònia Borrell
, Nicola Thorne
, Montserrat Español
, Chloe Mortimer
, Àngels Orcau
, Pere Coll
, Saheer Gharbia
, Julian González-Martín
, Catherine Arnold^*

^*Corresponding author for this work

Research, Evidence & Knowledge Divisional Office

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

The purpose of the study was to compare the IS6110-RFLP (RFLP) results obtained in a previous epidemiological study in the city of Barcelona, Spain [Borrell S, Espanol M, Orcau A, Tudo G, March F, Cayla JA, et al. Factors associated with differences between conventional contact tracing and molecular epidemiology in study of tuberculosis transmission and analysis in the city of Barcelona, Spain. J Clin Microbiol 2009 Jan;47(1):198-204.] with the results obtained with IS6110-fAFLP, [Thorne N, Evans JT, Smith EG, Hawkey PM, Gharbia S, Arnold C. An IS6110-targeting fluorescent amplified fragment length polymorphism alternative to IS6110-restriction fragment length polymorphism analysis for Mycobacterium tuberculosis DNA fingerprinting. Clin Microbiol Infect 2007 Oct;13(10):964-70.] on the ability to detect recent transmission. fAFLP was applied to DNA samples of RFLP clustered strains, with and without known epidemiological links, with the additional inclusion of four nucleotide-specific fluorophores to further increase the discrimination of the fragments obtained. Four-colour fAFLP was performed on 123 RFLP clustered strains with no epidemiological link (NELC) and on 28 epidemiologically linked RFLP clustered (ELC) strains grouped into 48 and 13 clusters respectively. Clustering results obtained by the two methods were highly congruent in ELC strains with fAFLP allocating 92.3% of the ELCs. For the NELCs, RFLP results were confirmed in 39/48 (81.2%) of fAFLP-clusters with 0-1 different fragments and 9/48 (18.8%) differed in 2-4 fragments, which are considered genetically related but not recently transmitted. In conclusion, overestimation of recent tuberculosis transmission can occur because of the inaccurate analysis of RFLP results. Four-colour fAFLP allows us to differentiate between recent transmission strains and epidemiologically unrelated but genetically related strains.

Original language	English
Pages (from-to)	233-237
Number of pages	5
Journal	Tuberculosis
Volume	89
Issue number	3
DOIs	https://doi.org/10.1016/j.tube.2009.03.003
Publication status	Published - May 2009

Bibliographical note

Funding Information:
This study was financed by grants from Fondo de Investigaciones Sanitarias (02/1489, 02/0348, 04/2381), a grant from la Fundació La Marató de TV3 and was supported by Ministerio de Sanidad y Consumo, Instituto de Salud Carlos III and the Spanish Network for the Research in Infectious Diseases (REIPI RD06/0008) and CIBER Enfermedades Respiratorias, Instituto de Salud Carlos III. S.B. received a grant of Formation in Research and Teaching (BRD) from the University of Barcelona.

Keywords

Fluorescent AFLP analysis
IS6110
Molecular typing
Mycobacterium tuberculosis
RFLP

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.tube.2009.03.003

Cite this

@article{3e21498f3d4746e99c40ce485cb2672a,

title = "Comparison of four-colour IS6110-fAFLP with the classic IS6110-RFLP on the ability to detect recent transmission in the city of Barcelona, Spain",

abstract = "The purpose of the study was to compare the IS6110-RFLP (RFLP) results obtained in a previous epidemiological study in the city of Barcelona, Spain [Borrell S, Espanol M, Orcau A, Tudo G, March F, Cayla JA, et al. Factors associated with differences between conventional contact tracing and molecular epidemiology in study of tuberculosis transmission and analysis in the city of Barcelona, Spain. J Clin Microbiol 2009 Jan;47(1):198-204.] with the results obtained with IS6110-fAFLP, [Thorne N, Evans JT, Smith EG, Hawkey PM, Gharbia S, Arnold C. An IS6110-targeting fluorescent amplified fragment length polymorphism alternative to IS6110-restriction fragment length polymorphism analysis for Mycobacterium tuberculosis DNA fingerprinting. Clin Microbiol Infect 2007 Oct;13(10):964-70.] on the ability to detect recent transmission. fAFLP was applied to DNA samples of RFLP clustered strains, with and without known epidemiological links, with the additional inclusion of four nucleotide-specific fluorophores to further increase the discrimination of the fragments obtained. Four-colour fAFLP was performed on 123 RFLP clustered strains with no epidemiological link (NELC) and on 28 epidemiologically linked RFLP clustered (ELC) strains grouped into 48 and 13 clusters respectively. Clustering results obtained by the two methods were highly congruent in ELC strains with fAFLP allocating 92.3\% of the ELCs. For the NELCs, RFLP results were confirmed in 39/48 (81.2\%) of fAFLP-clusters with 0-1 different fragments and 9/48 (18.8\%) differed in 2-4 fragments, which are considered genetically related but not recently transmitted. In conclusion, overestimation of recent tuberculosis transmission can occur because of the inaccurate analysis of RFLP results. Four-colour fAFLP allows us to differentiate between recent transmission strains and epidemiologically unrelated but genetically related strains.",

keywords = "Fluorescent AFLP analysis, IS6110, Molecular typing, Mycobacterium tuberculosis, RFLP",

author = "S{\`o}nia Borrell and Nicola Thorne and Montserrat Espa{\~n}ol and Chloe Mortimer and {\`A}ngels Orcau and Pere Coll and Saheer Gharbia and Julian Gonz{\'a}lez-Mart{\'i}n and Catherine Arnold",

note = "Funding Information: This study was financed by grants from Fondo de Investigaciones Sanitarias (02/1489, 02/0348, 04/2381), a grant from la Fundaci{\'o} La Marat{\'o} de TV3 and was supported by Ministerio de Sanidad y Consumo, Instituto de Salud Carlos III and the Spanish Network for the Research in Infectious Diseases (REIPI RD06/0008) and CIBER Enfermedades Respiratorias, Instituto de Salud Carlos III. S.B. received a grant of Formation in Research and Teaching (BRD) from the University of Barcelona. ",

year = "2009",

month = may,

doi = "10.1016/j.tube.2009.03.003",

language = "English",

volume = "89",

pages = "233--237",

journal = "Tuberculosis",

issn = "1472-9792",

publisher = "Churchill Livingstone",

number = "3",

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TY - JOUR

T1 - Comparison of four-colour IS6110-fAFLP with the classic IS6110-RFLP on the ability to detect recent transmission in the city of Barcelona, Spain

AU - Borrell, Sònia

AU - Thorne, Nicola

AU - Español, Montserrat

AU - Mortimer, Chloe

AU - Orcau, Àngels

AU - Coll, Pere

AU - Gharbia, Saheer

AU - González-Martín, Julian

AU - Arnold, Catherine

N1 - Funding Information: This study was financed by grants from Fondo de Investigaciones Sanitarias (02/1489, 02/0348, 04/2381), a grant from la Fundació La Marató de TV3 and was supported by Ministerio de Sanidad y Consumo, Instituto de Salud Carlos III and the Spanish Network for the Research in Infectious Diseases (REIPI RD06/0008) and CIBER Enfermedades Respiratorias, Instituto de Salud Carlos III. S.B. received a grant of Formation in Research and Teaching (BRD) from the University of Barcelona.

PY - 2009/5

Y1 - 2009/5

N2 - The purpose of the study was to compare the IS6110-RFLP (RFLP) results obtained in a previous epidemiological study in the city of Barcelona, Spain [Borrell S, Espanol M, Orcau A, Tudo G, March F, Cayla JA, et al. Factors associated with differences between conventional contact tracing and molecular epidemiology in study of tuberculosis transmission and analysis in the city of Barcelona, Spain. J Clin Microbiol 2009 Jan;47(1):198-204.] with the results obtained with IS6110-fAFLP, [Thorne N, Evans JT, Smith EG, Hawkey PM, Gharbia S, Arnold C. An IS6110-targeting fluorescent amplified fragment length polymorphism alternative to IS6110-restriction fragment length polymorphism analysis for Mycobacterium tuberculosis DNA fingerprinting. Clin Microbiol Infect 2007 Oct;13(10):964-70.] on the ability to detect recent transmission. fAFLP was applied to DNA samples of RFLP clustered strains, with and without known epidemiological links, with the additional inclusion of four nucleotide-specific fluorophores to further increase the discrimination of the fragments obtained. Four-colour fAFLP was performed on 123 RFLP clustered strains with no epidemiological link (NELC) and on 28 epidemiologically linked RFLP clustered (ELC) strains grouped into 48 and 13 clusters respectively. Clustering results obtained by the two methods were highly congruent in ELC strains with fAFLP allocating 92.3% of the ELCs. For the NELCs, RFLP results were confirmed in 39/48 (81.2%) of fAFLP-clusters with 0-1 different fragments and 9/48 (18.8%) differed in 2-4 fragments, which are considered genetically related but not recently transmitted. In conclusion, overestimation of recent tuberculosis transmission can occur because of the inaccurate analysis of RFLP results. Four-colour fAFLP allows us to differentiate between recent transmission strains and epidemiologically unrelated but genetically related strains.

AB - The purpose of the study was to compare the IS6110-RFLP (RFLP) results obtained in a previous epidemiological study in the city of Barcelona, Spain [Borrell S, Espanol M, Orcau A, Tudo G, March F, Cayla JA, et al. Factors associated with differences between conventional contact tracing and molecular epidemiology in study of tuberculosis transmission and analysis in the city of Barcelona, Spain. J Clin Microbiol 2009 Jan;47(1):198-204.] with the results obtained with IS6110-fAFLP, [Thorne N, Evans JT, Smith EG, Hawkey PM, Gharbia S, Arnold C. An IS6110-targeting fluorescent amplified fragment length polymorphism alternative to IS6110-restriction fragment length polymorphism analysis for Mycobacterium tuberculosis DNA fingerprinting. Clin Microbiol Infect 2007 Oct;13(10):964-70.] on the ability to detect recent transmission. fAFLP was applied to DNA samples of RFLP clustered strains, with and without known epidemiological links, with the additional inclusion of four nucleotide-specific fluorophores to further increase the discrimination of the fragments obtained. Four-colour fAFLP was performed on 123 RFLP clustered strains with no epidemiological link (NELC) and on 28 epidemiologically linked RFLP clustered (ELC) strains grouped into 48 and 13 clusters respectively. Clustering results obtained by the two methods were highly congruent in ELC strains with fAFLP allocating 92.3% of the ELCs. For the NELCs, RFLP results were confirmed in 39/48 (81.2%) of fAFLP-clusters with 0-1 different fragments and 9/48 (18.8%) differed in 2-4 fragments, which are considered genetically related but not recently transmitted. In conclusion, overestimation of recent tuberculosis transmission can occur because of the inaccurate analysis of RFLP results. Four-colour fAFLP allows us to differentiate between recent transmission strains and epidemiologically unrelated but genetically related strains.

KW - Fluorescent AFLP analysis

KW - IS6110

KW - Molecular typing

KW - Mycobacterium tuberculosis

KW - RFLP

UR - https://www.scopus.com/pages/publications/67349221462

U2 - 10.1016/j.tube.2009.03.003

DO - 10.1016/j.tube.2009.03.003

M3 - Article

C2 - 19409847

AN - SCOPUS:67349221462

SN - 1472-9792

VL - 89

SP - 233

EP - 237

JO - Tuberculosis

JF - Tuberculosis

IS - 3

ER -

Comparison of four-colour IS6110-fAFLP with the classic IS6110-RFLP on the ability to detect recent transmission in the city of Barcelona, Spain

Abstract

Bibliographical note

Keywords

UN SDGs

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