Comparison of Control of Clostridium difficile Infection in Six English Hospitals Using Whole-Genome Sequencing

David W. Eyre*, Warren N. Fawley, Anu Rajgopal, Christopher Settle, Kalani Mortimer, Simon D. Goldenberg, Susan Dawson, Derrick W. Crook, Tim E.A. Peto, A. Sarah Walker, Mark H. Wilcox

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

35 Citations (Scopus)


Background. Variation in Clostridium difficile infection (CDI) rates between healthcare institutions suggests overall incidence could be reduced if the lowest rates could be achieved more widely. Methods. We used whole-genome sequencing (WGS) of consecutive C. difficile isolates from 6 English hospitals over 1 year (2013-14) to compare infection control performance. Fecal samples with a positive initial screen for C. difficile were sequenced. Within each hospital, we estimated the proportion of cases plausibly acquired from previous cases. Results. Overall, 851/971 (87.6%) sequenced samples contained toxin genes, and 451 (46.4%) were fecal-toxin-positive. Of 652 potentially toxigenic isolates >90-days after the study started, 128 (20%, 95% confidence interval [CI] 17-23%) were genetically linked (within ≤2 single nucleotide polymorphisms) to a prior patient's isolate from the previous 90 days. Hospital 2 had the fewest linked isolates, 7/105 (7%, 3-13%), hospital 1, 9/70 (13%, 6-23%), and hospitals 3-6 had similar proportions of linked isolates (22-26%) (P ≤.002 comparing hospital-2 vs 3-6). Results were similar adjusting for locally circulating ribotypes. Adjusting for hospital, ribotype-027 had the highest proportion of linked isolates (57%, 95% CI 29-81%). Fecal-toxin-positive and toxin-negative patients were similarly likely to be a potential transmission donor, OR = 1.01 (0.68-1.49). There was no association between the estimated proportion of linked cases and testing rates. Conclusions. WGS can be used as a novel surveillance tool to identify varying rates of C. difficile transmission between institutions and therefore to allow targeted efforts to reduce CDI incidence.

Original languageEnglish
Pages (from-to)433-441
Number of pages9
JournalClinical Infectious Diseases
Issue number3
Publication statusPublished - 1 Aug 2017
Externally publishedYes

Bibliographical note

Publisher Copyright:
© The Author 2017.


  • Clostridium difficile
  • infection control
  • surveillance
  • transmission
  • whole-genome sequencing


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