Comparison of a frozen human foreskin fibroblast cell assay to an enzyme immunoassay and toxigenic culture for the detection of toxigenic Clostridium difficile

Alastair J. Strachan; Natalie E. Evans; O. Martin Williams; Robert C. Spencer; Rosemary Greenwood; Chris J. Probert

doi:10.1016/j.diagmicrobio.2012.09.013

Comparison of a frozen human foreskin fibroblast cell assay to an enzyme immunoassay and toxigenic culture for the detection of toxigenic Clostridium difficile

Alastair J. Strachan^*, Natalie E. Evans, O. Martin Williams, Robert C. Spencer, Rosemary Greenwood, Chris J. Probert

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

This study set out to validate the Hs27 ReadyCell assay (RCCNA) as an alternative CCNA method compared against a commonly used commercial enzyme immunoassay (EIA) method and toxigenic culture (TC) reference standard. A total of 860 samples were identified from those submitted to the Health Protection Agency microbiology laboratories over a 30-week period. RCCNA performed much better than EIA when using TC as a gold standard, with sensitivities of 90.8% versus 78.6% and positive predictive value of 87.3% to 81.9%, respectively. The Hs27 Human Foreskin Fibroblast ReadyCells are an easy-to-use and a sensitive CCNA method for the detection of toxigenic Clostridium difficile directly from stool. A turnaround time of up to 48 h for a negative result and possible need for repeat testing make it an unsuitable method to be used in most clinical laboratory setting.

Original language	English
Pages (from-to)	42-45
Number of pages	4
Journal	Diagnostic Microbiology and Infectious Disease
Volume	75
Issue number	1
DOIs	https://doi.org/10.1016/j.diagmicrobio.2012.09.013
Publication status	Published - Jan 2013

Keywords

Clostridium difficile
Cytotoxin
Enzyme immunoassay
Toxigenic culture

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.diagmicrobio.2012.09.013

Cite this

Strachan, A. J., Evans, N. E., Williams, O. M., Spencer, R. C., Greenwood, R., & Probert, C. J. (2013). Comparison of a frozen human foreskin fibroblast cell assay to an enzyme immunoassay and toxigenic culture for the detection of toxigenic Clostridium difficile. Diagnostic Microbiology and Infectious Disease, 75(1), 42-45. https://doi.org/10.1016/j.diagmicrobio.2012.09.013

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title = "Comparison of a frozen human foreskin fibroblast cell assay to an enzyme immunoassay and toxigenic culture for the detection of toxigenic Clostridium difficile",

abstract = "This study set out to validate the Hs27 ReadyCell assay (RCCNA) as an alternative CCNA method compared against a commonly used commercial enzyme immunoassay (EIA) method and toxigenic culture (TC) reference standard. A total of 860 samples were identified from those submitted to the Health Protection Agency microbiology laboratories over a 30-week period. RCCNA performed much better than EIA when using TC as a gold standard, with sensitivities of 90.8% versus 78.6% and positive predictive value of 87.3% to 81.9%, respectively. The Hs27 Human Foreskin Fibroblast ReadyCells are an easy-to-use and a sensitive CCNA method for the detection of toxigenic Clostridium difficile directly from stool. A turnaround time of up to 48 h for a negative result and possible need for repeat testing make it an unsuitable method to be used in most clinical laboratory setting.",

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author = "Strachan, {Alastair J.} and Evans, {Natalie E.} and Williams, {O. Martin} and Spencer, {Robert C.} and Rosemary Greenwood and Probert, {Chris J.}",

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Strachan, AJ, Evans, NE, Williams, OM, Spencer, RC, Greenwood, R & Probert, CJ 2013, 'Comparison of a frozen human foreskin fibroblast cell assay to an enzyme immunoassay and toxigenic culture for the detection of toxigenic Clostridium difficile', Diagnostic Microbiology and Infectious Disease, vol. 75, no. 1, pp. 42-45. https://doi.org/10.1016/j.diagmicrobio.2012.09.013

Comparison of a frozen human foreskin fibroblast cell assay to an enzyme immunoassay and toxigenic culture for the detection of toxigenic Clostridium difficile. / Strachan, Alastair J.; Evans, Natalie E.; Williams, O. Martin et al.
In: Diagnostic Microbiology and Infectious Disease, Vol. 75, No. 1, 01.2013, p. 42-45.

Research output: Contribution to journal › Article › peer-review

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AU - Strachan, Alastair J.

AU - Evans, Natalie E.

AU - Williams, O. Martin

AU - Spencer, Robert C.

AU - Greenwood, Rosemary

AU - Probert, Chris J.

PY - 2013/1

Y1 - 2013/1

N2 - This study set out to validate the Hs27 ReadyCell assay (RCCNA) as an alternative CCNA method compared against a commonly used commercial enzyme immunoassay (EIA) method and toxigenic culture (TC) reference standard. A total of 860 samples were identified from those submitted to the Health Protection Agency microbiology laboratories over a 30-week period. RCCNA performed much better than EIA when using TC as a gold standard, with sensitivities of 90.8% versus 78.6% and positive predictive value of 87.3% to 81.9%, respectively. The Hs27 Human Foreskin Fibroblast ReadyCells are an easy-to-use and a sensitive CCNA method for the detection of toxigenic Clostridium difficile directly from stool. A turnaround time of up to 48 h for a negative result and possible need for repeat testing make it an unsuitable method to be used in most clinical laboratory setting.

AB - This study set out to validate the Hs27 ReadyCell assay (RCCNA) as an alternative CCNA method compared against a commonly used commercial enzyme immunoassay (EIA) method and toxigenic culture (TC) reference standard. A total of 860 samples were identified from those submitted to the Health Protection Agency microbiology laboratories over a 30-week period. RCCNA performed much better than EIA when using TC as a gold standard, with sensitivities of 90.8% versus 78.6% and positive predictive value of 87.3% to 81.9%, respectively. The Hs27 Human Foreskin Fibroblast ReadyCells are an easy-to-use and a sensitive CCNA method for the detection of toxigenic Clostridium difficile directly from stool. A turnaround time of up to 48 h for a negative result and possible need for repeat testing make it an unsuitable method to be used in most clinical laboratory setting.

KW - Clostridium difficile

KW - Cytotoxin

KW - Enzyme immunoassay

KW - Toxigenic culture

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ER -

Comparison of a frozen human foreskin fibroblast cell assay to an enzyme immunoassay and toxigenic culture for the detection of toxigenic Clostridium difficile

Abstract

Keywords

UN SDGs

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