Comparison of 2 chromogenic media for the detection of extended-spectrum β-lactamase producing Enterobacteriaceae stool carriage in nursing home residents

Beth Blane*, Hayley J. Brodrick, Theodore Gouliouris, Kirsty E. Ambridge, Angela D. Kidney, Catherine M. Ludden, Direk Limmathurotsakul, M. Estée Török, Sharon Peacock

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    7 Citations (Scopus)

    Abstract

    ChromID ESBL agar and Brilliance ESBL agar were compared for the isolation of extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae from 298 stools. These had comparable sensitivity and selectivity for the 116 positive samples. Pre-enrichment with cefpodoxime and extending incubation to 48 hours after direct plating both significantly increased sensitivity but reduced selectivity of both agars.

    Original languageEnglish
    Pages (from-to)181-183
    Number of pages3
    JournalDiagnostic Microbiology and Infectious Disease
    Volume84
    Issue number3
    DOIs
    Publication statusPublished - 1 Mar 2016

    Bibliographical note

    Funding Information:
    This publication presents independent research supported by the Health Innovation Challenge Fund ( WT098600 , HICF-T5-342 ), a parallel funding partnership between the Department of Health and Wellcome Trust. The views expressed in this publication are those of the author(s) and not necessarily those of the Department of Health or Wellcome Trust. TG is a Wellcome TrustResearch Training Fellow. MET is a Clinician Scientist Fellow, supported.

    Publisher Copyright:
    © 2016 The Authors.

    Keywords

    • Chromogenic agar
    • Detection
    • Enterobacteriaceae
    • Extended-spectrum β-lactamase
    • Selectivity
    • Sensitivity

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