Comparative genomics and DNA array-based genotyping of pandemic Staphylococcus aureus strains encoding Panton-Valentine leukocidin

S. Monecke; B. Berger-Bächi; G. Coombs; A. Holmes; I. Kay; Angela Kearns; H. J. Linde; F. O'Brien; P. Slickers; R. Ehricht

doi:10.1111/j.1469-0691.2006.01635.x

Comparative genomics and DNA array-based genotyping of pandemic Staphylococcus aureus strains encoding Panton-Valentine leukocidin

S. Monecke^*, B. Berger-Bächi, G. Coombs, A. Holmes, I. Kay, Angela Kearns, H. J. Linde, F. O'Brien, P. Slickers, R. Ehricht

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

89 Citations (Scopus)

Abstract

Within the last few years, methicillin-resistant Staphylococcus aureus (MRSA) strains encoding Panton-Valentine leukocidin (PVL) have emerged and spread worldwide. This epidemic can be attributed to a small number of distinct clones. The present study used a novel assay, based on multiplex linear DNA amplification and subsequent microarray hybridisation, to simultaneously detect all relevant exotoxins, antimicrobial resistance determinants and the allelic variants of agr. The genes of the staphylococcal exotoxin-like (set) locus were also included for typing purposes. This assay, together with multilocus sequence typing (MLST) and spa typing, was applied to 56 clinical isolates and reference strains representing all major pandemic PVL-MRSA lineages, as well as to phylogenetically-related strains and putative ancestors. Array hybridisation results allowed the assignment of isolates to clonal groups, which were in accordance with MLST and spa typing data. Ten distinct clonal groups of PVL-MRSA (ST1, ST5, ST8, ST22, ST30, ST59/359, ST80/583, ST88, ST93 and ST152), including 12 MLST types, were identified and analysed with regard to resistance determinants and genes coding for exotoxins. The array hybridisation data confirmed that pandemic PVL-positive strains originate from very diverse genetic backgrounds, and provided insights into the evolution of some lineages. The DNA microarray technique provides a valuable epidemiological tool for the detailed characterisation of clinical isolates and comparison of strains at a global level.

Original language	English
Pages (from-to)	236-249
Number of pages	14
Journal	Clinical Microbiology and Infection
Volume	13
Issue number	3
DOIs	https://doi.org/10.1111/j.1469-0691.2006.01635.x
Publication status	Published - Mar 2007

Bibliographical note

Funding Information:
Part of this work was supported by a grant from the Technical University of Dresden. Reference strains were provided by the Network on Antimicrobial Resistance in Staphylococcus aureus (NARSA) and by the Institut Pasteur, Paris. We acknowledge helpful discussions with E. Jacobs and W. Rudolph. The authors thank all clinical colleagues who referred patients or submitted isolates. We are indebted to A. Ruppelt, M. Ganner, S. Kolewa, I. Engelmann, I. McCormick-Smith, E. MÜller, T. Pryce and J. Sachtschal for technical assistance. R. Ehricht and P. Slickers are employees of CLONDIAG chip technologies.

Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.

Keywords

Community-associated MRSA
DNA microarray
Evolution
Exotoxins
Methicillin-resistant Staphylococcus aureus
Panton-Valentine leukocidin

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1111/j.1469-0691.2006.01635.x

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Monecke, S., Berger-Bächi, B., Coombs, G., Holmes, A., Kay, I., Kearns, A., Linde, H. J., O'Brien, F., Slickers, P., & Ehricht, R. (2007). Comparative genomics and DNA array-based genotyping of pandemic Staphylococcus aureus strains encoding Panton-Valentine leukocidin. Clinical Microbiology and Infection, 13(3), 236-249. https://doi.org/10.1111/j.1469-0691.2006.01635.x

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abstract = "Within the last few years, methicillin-resistant Staphylococcus aureus (MRSA) strains encoding Panton-Valentine leukocidin (PVL) have emerged and spread worldwide. This epidemic can be attributed to a small number of distinct clones. The present study used a novel assay, based on multiplex linear DNA amplification and subsequent microarray hybridisation, to simultaneously detect all relevant exotoxins, antimicrobial resistance determinants and the allelic variants of agr. The genes of the staphylococcal exotoxin-like (set) locus were also included for typing purposes. This assay, together with multilocus sequence typing (MLST) and spa typing, was applied to 56 clinical isolates and reference strains representing all major pandemic PVL-MRSA lineages, as well as to phylogenetically-related strains and putative ancestors. Array hybridisation results allowed the assignment of isolates to clonal groups, which were in accordance with MLST and spa typing data. Ten distinct clonal groups of PVL-MRSA (ST1, ST5, ST8, ST22, ST30, ST59/359, ST80/583, ST88, ST93 and ST152), including 12 MLST types, were identified and analysed with regard to resistance determinants and genes coding for exotoxins. The array hybridisation data confirmed that pandemic PVL-positive strains originate from very diverse genetic backgrounds, and provided insights into the evolution of some lineages. The DNA microarray technique provides a valuable epidemiological tool for the detailed characterisation of clinical isolates and comparison of strains at a global level.",

keywords = "Community-associated MRSA, DNA microarray, Evolution, Exotoxins, Methicillin-resistant Staphylococcus aureus, Panton-Valentine leukocidin",

author = "S. Monecke and B. Berger-B{\"a}chi and G. Coombs and A. Holmes and I. Kay and Angela Kearns and Linde, {H. J.} and F. O'Brien and P. Slickers and R. Ehricht",

note = "Funding Information: Part of this work was supported by a grant from the Technical University of Dresden. Reference strains were provided by the Network on Antimicrobial Resistance in Staphylococcus aureus (NARSA) and by the Institut Pasteur, Paris. We acknowledge helpful discussions with E. Jacobs and W. Rudolph. The authors thank all clinical colleagues who referred patients or submitted isolates. We are indebted to A. Ruppelt, M. Ganner, S. Kolewa, I. Engelmann, I. McCormick-Smith, E. M{\"U}ller, T. Pryce and J. Sachtschal for technical assistance. R. Ehricht and P. Slickers are employees of CLONDIAG chip technologies. Copyright: Copyright 2017 Elsevier B.V., All rights reserved.",

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Monecke, S, Berger-Bächi, B, Coombs, G, Holmes, A, Kay, I, Kearns, A, Linde, HJ, O'Brien, F, Slickers, P & Ehricht, R 2007, 'Comparative genomics and DNA array-based genotyping of pandemic Staphylococcus aureus strains encoding Panton-Valentine leukocidin', Clinical Microbiology and Infection, vol. 13, no. 3, pp. 236-249. https://doi.org/10.1111/j.1469-0691.2006.01635.x

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T1 - Comparative genomics and DNA array-based genotyping of pandemic Staphylococcus aureus strains encoding Panton-Valentine leukocidin

AU - Monecke, S.

AU - Berger-Bächi, B.

AU - Coombs, G.

AU - Holmes, A.

AU - Kay, I.

AU - Kearns, Angela

AU - Linde, H. J.

AU - O'Brien, F.

AU - Slickers, P.

AU - Ehricht, R.

N1 - Funding Information: Part of this work was supported by a grant from the Technical University of Dresden. Reference strains were provided by the Network on Antimicrobial Resistance in Staphylococcus aureus (NARSA) and by the Institut Pasteur, Paris. We acknowledge helpful discussions with E. Jacobs and W. Rudolph. The authors thank all clinical colleagues who referred patients or submitted isolates. We are indebted to A. Ruppelt, M. Ganner, S. Kolewa, I. Engelmann, I. McCormick-Smith, E. MÜller, T. Pryce and J. Sachtschal for technical assistance. R. Ehricht and P. Slickers are employees of CLONDIAG chip technologies. Copyright: Copyright 2017 Elsevier B.V., All rights reserved.

PY - 2007/3

Y1 - 2007/3

N2 - Within the last few years, methicillin-resistant Staphylococcus aureus (MRSA) strains encoding Panton-Valentine leukocidin (PVL) have emerged and spread worldwide. This epidemic can be attributed to a small number of distinct clones. The present study used a novel assay, based on multiplex linear DNA amplification and subsequent microarray hybridisation, to simultaneously detect all relevant exotoxins, antimicrobial resistance determinants and the allelic variants of agr. The genes of the staphylococcal exotoxin-like (set) locus were also included for typing purposes. This assay, together with multilocus sequence typing (MLST) and spa typing, was applied to 56 clinical isolates and reference strains representing all major pandemic PVL-MRSA lineages, as well as to phylogenetically-related strains and putative ancestors. Array hybridisation results allowed the assignment of isolates to clonal groups, which were in accordance with MLST and spa typing data. Ten distinct clonal groups of PVL-MRSA (ST1, ST5, ST8, ST22, ST30, ST59/359, ST80/583, ST88, ST93 and ST152), including 12 MLST types, were identified and analysed with regard to resistance determinants and genes coding for exotoxins. The array hybridisation data confirmed that pandemic PVL-positive strains originate from very diverse genetic backgrounds, and provided insights into the evolution of some lineages. The DNA microarray technique provides a valuable epidemiological tool for the detailed characterisation of clinical isolates and comparison of strains at a global level.

AB - Within the last few years, methicillin-resistant Staphylococcus aureus (MRSA) strains encoding Panton-Valentine leukocidin (PVL) have emerged and spread worldwide. This epidemic can be attributed to a small number of distinct clones. The present study used a novel assay, based on multiplex linear DNA amplification and subsequent microarray hybridisation, to simultaneously detect all relevant exotoxins, antimicrobial resistance determinants and the allelic variants of agr. The genes of the staphylococcal exotoxin-like (set) locus were also included for typing purposes. This assay, together with multilocus sequence typing (MLST) and spa typing, was applied to 56 clinical isolates and reference strains representing all major pandemic PVL-MRSA lineages, as well as to phylogenetically-related strains and putative ancestors. Array hybridisation results allowed the assignment of isolates to clonal groups, which were in accordance with MLST and spa typing data. Ten distinct clonal groups of PVL-MRSA (ST1, ST5, ST8, ST22, ST30, ST59/359, ST80/583, ST88, ST93 and ST152), including 12 MLST types, were identified and analysed with regard to resistance determinants and genes coding for exotoxins. The array hybridisation data confirmed that pandemic PVL-positive strains originate from very diverse genetic backgrounds, and provided insights into the evolution of some lineages. The DNA microarray technique provides a valuable epidemiological tool for the detailed characterisation of clinical isolates and comparison of strains at a global level.

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KW - DNA microarray

KW - Evolution

KW - Exotoxins

KW - Methicillin-resistant Staphylococcus aureus

KW - Panton-Valentine leukocidin

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Comparative genomics and DNA array-based genotyping of pandemic Staphylococcus aureus strains encoding Panton-Valentine leukocidin

Abstract

Bibliographical note

Keywords

UN SDGs

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