TY - JOUR
T1 - Clostridium difficile ribotype 126 in southern Taiwan
T2 - A cluster of three symptomatic cases
AU - Hung, Yuan Pin
AU - Lin, Hsiao Ju
AU - Tsai, Bo Yang
AU - Liu, Hsiao Chieh
AU - Liu, Hsiu Chuan
AU - Lee, Jen Chieh
AU - Wu, Yi Hui
AU - Wilcox, Mark H.
AU - Fawley, Warren N.
AU - Hsueh, Po Ren
AU - Tsai, Pei Jane
AU - Ko, Wen Chien
N1 - Publisher Copyright:
© 2014 Elsevier Ltd.
PY - 2014/12/1
Y1 - 2014/12/1
N2 - Introduction: Several virulent Clostridium difficile clones, designated as polymerase chain reaction (PCR) ribotypes 017, 027, or 078, are well recognized in western countries. However, the ribotype distribution of clinical C.difficile isolates in Taiwan remains unclear. Method: Between 2010 and 2012, we identified three patients with C.difficile-associated diarrhea (CDAD) at a hospital in southern Taiwan. The C.difficile strains isolated from these patients were further characterized by PCR detection of tcdA, tcdB, tcdC, cdtA, and cdtB, toxinotyping, multilocus sequence typing, ribotyping and repetitive-based PCR. Results: Three C.difficile strains harbored tcdCδ39 and belonged to multilocus sequence typing 11 (ST11), toxinotype V, and ribotype 126 (a ribotype 078-like clone). Notably, one patient developed pseudomembranous colitis and recurrent CDAD. These three isolates were noted between January 2012 and June 2012 and were identical, as evidenced by repetitive sequence-based PCR, suggestive of case clustering. Conclusion: A hypervirulent C.difficile clone, ribotype 126, causing pseudomembranous colitis and recurrent CDAD, is present in southern Taiwan.
AB - Introduction: Several virulent Clostridium difficile clones, designated as polymerase chain reaction (PCR) ribotypes 017, 027, or 078, are well recognized in western countries. However, the ribotype distribution of clinical C.difficile isolates in Taiwan remains unclear. Method: Between 2010 and 2012, we identified three patients with C.difficile-associated diarrhea (CDAD) at a hospital in southern Taiwan. The C.difficile strains isolated from these patients were further characterized by PCR detection of tcdA, tcdB, tcdC, cdtA, and cdtB, toxinotyping, multilocus sequence typing, ribotyping and repetitive-based PCR. Results: Three C.difficile strains harbored tcdCδ39 and belonged to multilocus sequence typing 11 (ST11), toxinotype V, and ribotype 126 (a ribotype 078-like clone). Notably, one patient developed pseudomembranous colitis and recurrent CDAD. These three isolates were noted between January 2012 and June 2012 and were identical, as evidenced by repetitive sequence-based PCR, suggestive of case clustering. Conclusion: A hypervirulent C.difficile clone, ribotype 126, causing pseudomembranous colitis and recurrent CDAD, is present in southern Taiwan.
KW - Clostridium difficile
KW - Ribotype 078-like
KW - Ribotype 126
KW - Taiwan
UR - http://www.scopus.com/inward/record.url?scp=84913530524&partnerID=8YFLogxK
U2 - 10.1016/j.anaerobe.2014.06.005
DO - 10.1016/j.anaerobe.2014.06.005
M3 - Article
C2 - 24956433
AN - SCOPUS:84913530524
SN - 1075-9964
VL - 30
SP - 188
EP - 192
JO - Anaerobe
JF - Anaerobe
ER -
