Cloning and sequence analysis of the genes encoding phoshotransbutyrylase and butyrate kinase from Clostridium acetobutylicum NCIMB 8052

John D. Oultram; lan D. Burr; Michael J. Elmore; Nigel P. Minton

doi:10.1016/0378-1119(93)90677-U

Cloning and sequence analysis of the genes encoding phoshotransbutyrylase and butyrate kinase from Clostridium acetobutylicum NCIMB 8052

John D. Oultram^*, lan D. Burr, Michael J. Elmore, Nigel P. Minton

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

22 Citations (Scopus)

Abstract

An 8.1-kb fragment of chromosomal DNA from Clostridium acetobutylicum NCIMB 8052 (formerly NCIB 8052) has been cloned into plasmid pAT153 and shown to allow the growth of Escherichia coli LJ32 (F⁺ atoC2^c atoD32 fadR) on butyrate as the sole source of carbon and energy. Deletion analysis delineated a 3.9-kb subfragment capable of complementation. The nucleotide sequence of this fragment was determined and it was shown to encode three complete, and two incomplete open reading frames (ORFs). Based on enzymic studies of recombinant clones, two of these ORFs were shown to encode phosphotransbutyrylase and butyrate kinase. The above enzymes are involved in the acidogenic phase of fermentation in C. acetobutylicum. The fragment also carries an incomplete ORF encoding a polypeptide exhibiting substantial similarity to dihydropteroate synthase.

Original language	English
Pages (from-to)	107-112
Number of pages	6
Journal	Gene
Volume	131
Issue number	1
DOIs	https://doi.org/10.1016/0378-1119(93)90677-U
Publication status	Published - 6 Sept 1993

Keywords

Recombinant DNA
acidogenesis
dihydropteroate synthetase

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title = "Cloning and sequence analysis of the genes encoding phoshotransbutyrylase and butyrate kinase from Clostridium acetobutylicum NCIMB 8052",

abstract = "An 8.1-kb fragment of chromosomal DNA from Clostridium acetobutylicum NCIMB 8052 (formerly NCIB 8052) has been cloned into plasmid pAT153 and shown to allow the growth of Escherichia coli LJ32 (F+ atoC2c atoD32 fadR) on butyrate as the sole source of carbon and energy. Deletion analysis delineated a 3.9-kb subfragment capable of complementation. The nucleotide sequence of this fragment was determined and it was shown to encode three complete, and two incomplete open reading frames (ORFs). Based on enzymic studies of recombinant clones, two of these ORFs were shown to encode phosphotransbutyrylase and butyrate kinase. The above enzymes are involved in the acidogenic phase of fermentation in C. acetobutylicum. The fragment also carries an incomplete ORF encoding a polypeptide exhibiting substantial similarity to dihydropteroate synthase.",

keywords = "Recombinant DNA, acidogenesis, dihydropteroate synthetase",

author = "Oultram, {John D.} and Burr, {lan D.} and Elmore, {Michael J.} and Minton, {Nigel P.}",

year = "1993",

month = sep,

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doi = "10.1016/0378-1119(93)90677-U",

language = "English",

volume = "131",

pages = "107--112",

journal = "Gene",

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T1 - Cloning and sequence analysis of the genes encoding phoshotransbutyrylase and butyrate kinase from Clostridium acetobutylicum NCIMB 8052

AU - Oultram, John D.

AU - Burr, lan D.

AU - Elmore, Michael J.

AU - Minton, Nigel P.

PY - 1993/9/6

Y1 - 1993/9/6

N2 - An 8.1-kb fragment of chromosomal DNA from Clostridium acetobutylicum NCIMB 8052 (formerly NCIB 8052) has been cloned into plasmid pAT153 and shown to allow the growth of Escherichia coli LJ32 (F+ atoC2c atoD32 fadR) on butyrate as the sole source of carbon and energy. Deletion analysis delineated a 3.9-kb subfragment capable of complementation. The nucleotide sequence of this fragment was determined and it was shown to encode three complete, and two incomplete open reading frames (ORFs). Based on enzymic studies of recombinant clones, two of these ORFs were shown to encode phosphotransbutyrylase and butyrate kinase. The above enzymes are involved in the acidogenic phase of fermentation in C. acetobutylicum. The fragment also carries an incomplete ORF encoding a polypeptide exhibiting substantial similarity to dihydropteroate synthase.

AB - An 8.1-kb fragment of chromosomal DNA from Clostridium acetobutylicum NCIMB 8052 (formerly NCIB 8052) has been cloned into plasmid pAT153 and shown to allow the growth of Escherichia coli LJ32 (F+ atoC2c atoD32 fadR) on butyrate as the sole source of carbon and energy. Deletion analysis delineated a 3.9-kb subfragment capable of complementation. The nucleotide sequence of this fragment was determined and it was shown to encode three complete, and two incomplete open reading frames (ORFs). Based on enzymic studies of recombinant clones, two of these ORFs were shown to encode phosphotransbutyrylase and butyrate kinase. The above enzymes are involved in the acidogenic phase of fermentation in C. acetobutylicum. The fragment also carries an incomplete ORF encoding a polypeptide exhibiting substantial similarity to dihydropteroate synthase.

KW - Recombinant DNA

KW - acidogenesis

KW - dihydropteroate synthetase

UR - http://www.scopus.com/inward/record.url?scp=0027268130&partnerID=8YFLogxK

U2 - 10.1016/0378-1119(93)90677-U

DO - 10.1016/0378-1119(93)90677-U

M3 - Article

C2 - 8396545

AN - SCOPUS:0027268130

SN - 0378-1119

VL - 131

SP - 107

EP - 112

JO - Gene

JF - Gene

IS - 1

ER -

Cloning and sequence analysis of the genes encoding phoshotransbutyrylase and butyrate kinase from Clostridium acetobutylicum NCIMB 8052

Abstract

Keywords

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