Clinical isolates of Staphylococcus aureus exhibit diversity in fnb genes and adhesion to human fibronectin

S. J. Peacock*, N. P.J. Day, M. G. Thomas, A. R. Berendt, T. J. Foster

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

129 Citations (Scopus)

Abstract

Objectives: The fibronectin-binding proteins (FnBPs) of Staphylococcus aureus are involved in the pathogenesis of infection, but their characteristics in clinical isolates are incompletely defined. The aim of this study was to evaluate phenotypic and genotypic characteristics of the FnBPs of a large collection of recent isolates. Methods: The adherence of 163 S. aureus isolates to immobilized fibronectin was compared with that of S. aureus 8325-4 using a microtitre assay. The presence of the genes encoding the fibronectin-binding proteins FnBPA and FnBPB was evaluated by Southern dot blot using probes specific for region A of fnbA or fnbB. Results: The adherence of clinical isolates to fibronectin (expressed as a percentage of the mean adherence of S. aureus 8325-4) was 56%-125% for 155 isolates (95%), and less than 20% for eight isolates (5%). Adherence of the bacterial group associated with orthopaedic implant-associated infection was significantly greater than that for isolates associated with nasal carriage, endocarditis, or septic arthritis/osteomyelitis. Southern dot blot demonstrated that 126/163 isolates had two genes (77%) and 37/163 had one detectable gene (23%). There was no difference in adherence between isolates with one or two fnb, but isolates associated with invasive disease (endocarditis or primary septic arthritis and/or osteomyelitis) were more likely to have two genes. Conclusions: These data demonstrate diversity in the FnBPs of clinical isolates of S. aureus. The findings suggest that the interplay between pathogenesis and a single virulence determinant is unlikely to be a uniform process across a spectrum of infections. This confirms the need to extend the study of staphylococcal pathogenesis from the laboratory to non-uniform populations of clinically relevant isolates. (C) 2000 The British Infection Society.

Original languageEnglish
Pages (from-to)23-31
Number of pages9
JournalJournal of Infection
Volume41
Issue number1
DOIs
Publication statusPublished - 2000
Externally publishedYes

Bibliographical note

Funding Information:
We are grateful to Drs Derrick Crook and Ian Bowler of the Oxford microbiology laboratory for providing clinical isolates of S. aureus. During this work, S. P. was a Wellcome Microbiology Training fellow (grant 044331), A.B. was a Lister Institute Research Fellow, and support was provided to M.T. by the Sir Harcourt Caughey Fund. N.D. is a Wellcome Trust Career Development Fellow in Tropical Medicine. This study was also supported by Wellcome Trust grant 52320, and BioResearch Ireland.

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