Abstract
The G2 chromosomal radiosensitivity assay is a technically demanding assay. To ensure that it is reproducible in our laboratory, we have examined the effects of storage and culture conditions by applying the assay to a group of healthy controls and determined the extent of intra- and inter-individual variations. Nineteen different individuals provided one or more blood samples resulting in a total of 57 successful tests. Multiple cultures from a single blood sample showed no statistically significant difference in the number of chromatid type aberrations between cultures. A 24 h delay prior to culturing the lymphocytes did not significantly affect the induced G2 score. Intra-individual variation was not statistically significant in seven out of nine individuals. Inter-individual variation was highly statistically significant (P < 0.001), indicating that there is a real difference between individuals in the response to radiation using this assay.
| Original language | English |
|---|---|
| Pages (from-to) | 105-110 |
| Number of pages | 6 |
| Journal | Mutation Research |
| Volume | 528 |
| Issue number | 1-2 |
| DOIs | |
| Publication status | Published - 25 Jul 2003 |
Bibliographical note
Funding Information:The authors thank all the individuals for donations of blood and Ms. Pat Jonas, West Cumberland Hospital, for collection of the samples. This work was supported by British Nuclear Fuels plc.
Keywords
- Chromosomal aberrations
- G chromosomal assay
- Lymphocytes
- Radiosensitivity