Characterization of plasmids encoding extended-spectrum β-lactamases and their addiction systems circulating among Escherichia coli clinical isolates in the UK

Michel Doumith*, Hiran Dhanji, Matthew J. Ellington, Peter Hawkey, Neil Woodford

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    69 Citations (Scopus)

    Abstract

    Objectives: To characterize plasmids encoding extended-spectrum β-lactamases (ESBLs) from a recent UK collection of clinical Escherichia coli isolates. Methods: The isolates comprised 118 ESBL producers referred from 54 laboratories. Plasmids were transferred by electroporation, and their incompatibility groups, associated addiction systems and resistance genes with the flanking genetic environments were identified by PCR or sequencing. Results: Seventy isolates had plasmids encoding CTX-M-15 (n = 53), CTX-M-14 (n = 9), CTX-M-27 (n = 1), CTX-M-3 (n = 2) and SHV-12 (n = 5) ESBLs that were transformable; non-transformable ESBLs were mainly CTX-M enzymes (42/48). Most transformable bla CTX-M-15 genes (43/53) were harboured on single replicon or multireplicon IncF plasmids, with IncFIA4-FIB1-FII31 (n = 11) and IncFIA1-FII2 (n = 15) being most frequent; the latter included eight pEK499 plasmids, typical of UK epidemic strain A. Plasmids harbouring bla CTX-M-14 belonged variously to IncF, IncI1 and IncHI2 types, and 16 encoding CTX-M or SHV enzymes were non-typeable. Only IncF plasmid types carried the addiction systems sought and those with bla CTX-M-15 frequently harboured bla OXA-1 and aac(6')-Ib-cr, and often transferred trimethoprim and tetracycline resistance; those with bla CTX-M-14 encoded trimethoprim, sulphonamide, streptomycin and tetracycline resistance. Most ESBL genes were associated with the well-known mobile elements ISEcp1 and IS26, but nearly half (23/55) of the ISEcp1 sequences upstream of bla CTX-M-15 were interrupted by an IS26 at various positions. Conclusions: Most ESBLs (70/118) were encoded by transformable plasmids, although a sizable minority could not be transformed. The majority of transformable plasmids (51/70; 72.9%) were diverse multiresistant IncF types possessing multiple addiction systems. The spread of bla CTX-M-15 can be attributed not just to clonal expansion, but also to the horizontal dissemination of related plasmids.

    Original languageEnglish
    Article numberdkr553
    Pages (from-to)878-885
    Number of pages8
    JournalJournal of Antimicrobial Chemotherapy
    Volume67
    Issue number4
    DOIs
    Publication statusPublished - Apr 2012

    Bibliographical note

    Funding Information:
    This project was funded by the Health Protection Agency’s Strategic Research and Development Fund, project number 105037.

    Keywords

    • Multiresistance
    • Replicon typing
    • ST131
    • Toxin-antitoxin systems

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