Characterisation of Legionella pneumophila antigen in urine of guinea pigs and humans with Legionnaires' disease

Ann Williams; M. S. Lever

doi:10.1016/S0163-4453(95)92691-7

Characterisation of Legionella pneumophila antigen in urine of guinea pigs and humans with Legionnaires' disease

Ann Williams, M. S. Lever^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

22 Citations (Scopus)

Abstract

To study and develop the urinary antigen detection assay, urine was obtained from aerosol infected guinea pigs. The appearance of Legionella pneumophila antigen in guinea pig urine was then compared with that detected in urine from human Legionnaires' Disease (LD) cases. This study demonstrated that the antigen expressed in the experimental model of LD was of identical molecular weight, reacted almost identically in an ELISA based assay and in Western blots with the antigen found in human urine. The guinea pig urine can therefore be used as a reliable, consistent and controllable source of antigen for use in the urinary antigen assay.

Original language	English
Pages (from-to)	13-16
Number of pages	4
Journal	Journal of Infection
Volume	30
Issue number	1
DOIs	https://doi.org/10.1016/S0163-4453(95)92691-7
Publication status	Published - Jan 1995

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10.1016/S0163-4453(95)92691-7

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abstract = "To study and develop the urinary antigen detection assay, urine was obtained from aerosol infected guinea pigs. The appearance of Legionella pneumophila antigen in guinea pig urine was then compared with that detected in urine from human Legionnaires' Disease (LD) cases. This study demonstrated that the antigen expressed in the experimental model of LD was of identical molecular weight, reacted almost identically in an ELISA based assay and in Western blots with the antigen found in human urine. The guinea pig urine can therefore be used as a reliable, consistent and controllable source of antigen for use in the urinary antigen assay.",

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AB - To study and develop the urinary antigen detection assay, urine was obtained from aerosol infected guinea pigs. The appearance of Legionella pneumophila antigen in guinea pig urine was then compared with that detected in urine from human Legionnaires' Disease (LD) cases. This study demonstrated that the antigen expressed in the experimental model of LD was of identical molecular weight, reacted almost identically in an ELISA based assay and in Western blots with the antigen found in human urine. The guinea pig urine can therefore be used as a reliable, consistent and controllable source of antigen for use in the urinary antigen assay.

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Characterisation of Legionella pneumophila antigen in urine of guinea pigs and humans with Legionnaires' disease

Abstract

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