Campylobacter fetus subsp. testudinum subsp. nov., Isolated from humans and reptiles

Collette Fitzgerald*, Zheng Chao Tu, Mary Patrick, Tracy Stiles, Andrew Lawson, Monica Santovenia, Maarten J. Gilbert, Marcel Van Bergen, Kevin Joyce, Janet Pruckler, Steven Stroika, Birgitta Duim, William G. Miller, Vladimir Loparev, Jan C. Sinnige, Patricia I. Fields, Robert V. Tauxe, Martin J. Blaser, Jaap A. Wagenaar

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

53 Citations (Scopus)

Abstract

A polyphasic study was undertaken to determine the taxonomic position of 13 Campylobacter fetuslike strains from humans (n=8) and reptiles (n=5). The results of matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) MS and genomic data from sap analysis, 16S rRNA gene and hsp60 sequence comparison, pulsed-field gel electrophoresis, amplified fragment length polymorphism analysis, DNA-DNA hybridization and whole genome sequencing demonstrated that these strains are closely related to C. fetus but clearly differentiated from recognized subspecies of C. fetus. Therefore, this unique cluster of 13 strains represents a novel subspecies within the species C. fetus, for which the name Campylobacter fetus subsp. testudinum subsp. nov. is proposed, with strain 03-427T (=ATCC BAA-2539T=LMG 27499T) as the type strain. Although this novel taxon could not be differentiated from C. fetus subsp. fetus and C. fetus subsp. venerealis using conventional phenotypic tests, MALDI-TOF MS revealed the presence of multiple phenotypic biomarkers which distinguish Campylobacter fetus subsp. testudinum subsp. nov. from recognized subspecies of C. fetus.

Original languageEnglish
Pages (from-to)2944-2948
Number of pages5
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume64
DOIs
Publication statusPublished - 1 Sep 2014

Bibliographical note

Publisher Copyright:
© 2014 IUMS.

Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.

Fingerprint

Dive into the research topics of 'Campylobacter fetus subsp. testudinum subsp. nov., Isolated from humans and reptiles'. Together they form a unique fingerprint.

Cite this