BurkDiff: A real-time PCR allelic discrimination assay for burkholderia Pseudomallei and B. mallei

Jolene R. Bowers; David M. Engelthaler; Jennifer L. Ginther; Talima Pearson; Sharon J. Peacock; Apichai Tuanyok; David M. Wagner; Bart J. Currie; Paul S. Keim

doi:10.1371/journal.pone.0015413

BurkDiff: A real-time PCR allelic discrimination assay for burkholderia Pseudomallei and B. mallei

Jolene R. Bowers, David M. Engelthaler, Jennifer L. Ginther, Talima Pearson, Sharon J. Peacock, Apichai Tuanyok, David M. Wagner, Bart J. Currie, Paul S. Keim

Research output: Contribution to journal › Article › peer-review

36 Citations (Scopus)

Abstract

A real-time PCR assay, BurkDiff, was designed to target a unique conserved region in the B. pseudomallei and B. mallei genomes containing a SNP that differentiates the two species. Sensitivity and specificity were assessed by screening BurkDiff across 469 isolates of B. pseudomallei, 49 isolates of B. mallei, and 390 isolates of clinically relevant non-target species. Concordance of results with traditional speciation methods and no cross-reactivity to non-target species show BurkDiff is a robust, highly validated assay for the detection and differentiation of B. pseudomallei and B. mallei.

Original language	English
Article number	e15413
Journal	PLoS ONE
Volume	5
Issue number	11
DOIs	https://doi.org/10.1371/journal.pone.0015413
Publication status	Published - 2010
Externally published	Yes

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title = "BurkDiff: A real-time PCR allelic discrimination assay for burkholderia Pseudomallei and B. mallei",

abstract = "A real-time PCR assay, BurkDiff, was designed to target a unique conserved region in the B. pseudomallei and B. mallei genomes containing a SNP that differentiates the two species. Sensitivity and specificity were assessed by screening BurkDiff across 469 isolates of B. pseudomallei, 49 isolates of B. mallei, and 390 isolates of clinically relevant non-target species. Concordance of results with traditional speciation methods and no cross-reactivity to non-target species show BurkDiff is a robust, highly validated assay for the detection and differentiation of B. pseudomallei and B. mallei.",

author = "Bowers, {Jolene R.} and Engelthaler, {David M.} and Ginther, {Jennifer L.} and Talima Pearson and Peacock, {Sharon J.} and Apichai Tuanyok and Wagner, {David M.} and Currie, {Bart J.} and Keim, {Paul S.}",

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AU - Ginther, Jennifer L.

AU - Pearson, Talima

AU - Peacock, Sharon J.

AU - Tuanyok, Apichai

AU - Wagner, David M.

AU - Currie, Bart J.

AU - Keim, Paul S.

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BurkDiff: A real-time PCR allelic discrimination assay for burkholderia Pseudomallei and B. mallei

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