TY - JOUR
T1 - Biosafety and biosecurity requirements for Orientia spp. diagnosis and research
T2 - Recommendations for risk-based biocontainment, work practices and the case for reclassification to risk group 2
AU - Blacksell, Stuart D.
AU - Robinson, Matthew T.
AU - Newton, Paul N.
AU - Ruanchaimun, Soiratchaneekorn
AU - Salje, Jeanne
AU - Wangrangsimakul, Tri
AU - Wegner, Matthew D.
AU - Abdad, Mohammad Yazid
AU - Bennett, Allan
AU - Richards, Allen L.
AU - Stenos, John
AU - Day, Nicholas P.J.
N1 - Publisher Copyright:
© 2019 The Author(s).
PY - 2019/12/10
Y1 - 2019/12/10
N2 - Scrub typhus is an important arthropod-borne disease causing significant acute febrile illness by infection with Orientia spp. Using a risk-based approach, this review examines current practice, the evidence base and regulatory requirements regarding matters of biosafety and biosecurity, and presents the case for reclassification from Risk Group 3 to Risk Group 2 along with recommendations for safe working practices of risk-based activities during the manipulation of Orientia spp. in the laboratory. We recommend to reclassify Orientia spp. to Risk Group 2 based on the classification for RG2 pathogens as being moderate individual risk, low community risk. We recommend that low risk activities, can be performed within a biological safety cabinet located in a Biosafety Level (BSL) 2 core laboratory using standard personal protective equipment. But when the risk assessment indicates, such as high concentration and volume, or aerosol generation, then a higher biocontainment level is warranted. For, the majority of animal activities involving Orientia spp., Animal BSL 2 (ABSL2) is recommended however where high risk activities are performed including necropsies, Animal BSL (ABSL3) is recommended.
AB - Scrub typhus is an important arthropod-borne disease causing significant acute febrile illness by infection with Orientia spp. Using a risk-based approach, this review examines current practice, the evidence base and regulatory requirements regarding matters of biosafety and biosecurity, and presents the case for reclassification from Risk Group 3 to Risk Group 2 along with recommendations for safe working practices of risk-based activities during the manipulation of Orientia spp. in the laboratory. We recommend to reclassify Orientia spp. to Risk Group 2 based on the classification for RG2 pathogens as being moderate individual risk, low community risk. We recommend that low risk activities, can be performed within a biological safety cabinet located in a Biosafety Level (BSL) 2 core laboratory using standard personal protective equipment. But when the risk assessment indicates, such as high concentration and volume, or aerosol generation, then a higher biocontainment level is warranted. For, the majority of animal activities involving Orientia spp., Animal BSL 2 (ABSL2) is recommended however where high risk activities are performed including necropsies, Animal BSL (ABSL3) is recommended.
KW - Biocontainment
KW - Biosafety
KW - Orientia
KW - Risk group
KW - Scrub typhus
UR - http://www.scopus.com/inward/record.url?scp=85076339022&partnerID=8YFLogxK
U2 - 10.1186/s12879-019-4653-4
DO - 10.1186/s12879-019-4653-4
M3 - Article
C2 - 31822267
AN - SCOPUS:85076339022
SN - 1471-2334
VL - 19
JO - BMC Infectious Diseases
JF - BMC Infectious Diseases
IS - 1
M1 - 1044
ER -