TY - JOUR
T1 - Biometrical evaluation of the performance of the revised OECD Test Guideline 402 for assessing acute dermal toxicity
AU - Mielke, H.
AU - Strickland, J.
AU - Jacobs, Miriam
AU - Mehta, J. M.
N1 - Publisher Copyright:
© 2017
PY - 2017/10
Y1 - 2017/10
N2 - A comprehensive biometrical assessment was conducted to compare the performance of multiple test designs for acute dermal systemic toxicity to support the animal welfare update to the original OECD Test Guideline (TG) 402 for acute dermal toxicity. The test designs evaluated included: (1) two, three, or five animals per dose group (2) evident toxicity or lethality endpoints and (3) absence or presence of a one-animal sighting study. The revision of TG 402 respected the 3R principles (replace, reduce, refine) of animal testing. The results demonstrate that the TG 402 test design can be optimised with reduced animal numbers per test group, such that a scenario of two animals per group following a sighting study at a starting dose of 200 mg/kg bw (unless further information is available to better define the starting dose) would provide a classification which in most cases is conservative, without compromising both the statistical ability of the study to assess dermal toxicity, or the relevant classification outcome.
AB - A comprehensive biometrical assessment was conducted to compare the performance of multiple test designs for acute dermal systemic toxicity to support the animal welfare update to the original OECD Test Guideline (TG) 402 for acute dermal toxicity. The test designs evaluated included: (1) two, three, or five animals per dose group (2) evident toxicity or lethality endpoints and (3) absence or presence of a one-animal sighting study. The revision of TG 402 respected the 3R principles (replace, reduce, refine) of animal testing. The results demonstrate that the TG 402 test design can be optimised with reduced animal numbers per test group, such that a scenario of two animals per group following a sighting study at a starting dose of 200 mg/kg bw (unless further information is available to better define the starting dose) would provide a classification which in most cases is conservative, without compromising both the statistical ability of the study to assess dermal toxicity, or the relevant classification outcome.
KW - Acute dermal toxicity
KW - Animal welfare
KW - Biometric evaluation
KW - Globally Harmonized System (GHS)
KW - Test guideline
UR - http://www.scopus.com/inward/record.url?scp=85025105058&partnerID=8YFLogxK
U2 - 10.1016/j.yrtph.2017.07.007
DO - 10.1016/j.yrtph.2017.07.007
M3 - Article
C2 - 28709685
AN - SCOPUS:85025105058
VL - 89
SP - 26
EP - 39
JO - Regulatory Toxicology and Pharmacology
JF - Regulatory Toxicology and Pharmacology
SN - 0273-2300
ER -