BinaxNOW® - An immunochromatographic test for the diagnosis of human influenza viruses: Comparison with viral culture and polymerase chain reaction

S. Bhattacharya; H. Osman

doi:10.4103/0255-0857.76518

BinaxNOW® - An immunochromatographic test for the diagnosis of human influenza viruses: Comparison with viral culture and polymerase chain reaction

S. Bhattacharya, H. Osman^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

Purpose: A rapid test for influenza viruses (Binax NOW®) was evaluated. Materials and Methods: In season-1, 35 respiratory samples were tested retrospectively; in season-2, 45 samples were tested prospectively [gold-standard: viral culture in season-1, culture+ reverse transcriptase- polymerase chain reaction (RT-PCR) in season-2]. Results: Sensitivity for Binax for influenza A was 59.3 and 0% in season-1 and -2, respectively. Sensitivity was low for influenza B (33.3% in season-1, 26.1% in season-2). Samples having low viral load were more likely to have a negative Binax test. Specificity of Binax was high (100 and 94.7% with influenza A and B, respectively). Conclusion: Sensitivity information provided in the kit insert does not always reflect post licensure performance in clinical settings.

Original language	English
Pages (from-to)	19-21
Number of pages	3
Journal	Indian Journal of Medical Microbiology
Volume	29
Issue number	1
DOIs	https://doi.org/10.4103/0255-0857.76518
Publication status	Published - Jan 2011
Externally published	Yes

Keywords

Influenza
rapid antigen test
sensitivity

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10.4103/0255-0857.76518

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title = "BinaxNOW{\textregistered} - An immunochromatographic test for the diagnosis of human influenza viruses: Comparison with viral culture and polymerase chain reaction",

abstract = "Purpose: A rapid test for influenza viruses (Binax NOW{\textregistered}) was evaluated. Materials and Methods: In season-1, 35 respiratory samples were tested retrospectively; in season-2, 45 samples were tested prospectively [gold-standard: viral culture in season-1, culture+ reverse transcriptase- polymerase chain reaction (RT-PCR) in season-2]. Results: Sensitivity for Binax for influenza A was 59.3 and 0% in season-1 and -2, respectively. Sensitivity was low for influenza B (33.3% in season-1, 26.1% in season-2). Samples having low viral load were more likely to have a negative Binax test. Specificity of Binax was high (100 and 94.7% with influenza A and B, respectively). Conclusion: Sensitivity information provided in the kit insert does not always reflect post licensure performance in clinical settings.",

keywords = "Influenza, rapid antigen test, sensitivity",

author = "S. Bhattacharya and H. Osman",

year = "2011",

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doi = "10.4103/0255-0857.76518",

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volume = "29",

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journal = "Indian Journal of Medical Microbiology",

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BinaxNOW® - An immunochromatographic test for the diagnosis of human influenza viruses: Comparison with viral culture and polymerase chain reaction

Abstract

Keywords

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