Assessment of Rapid Diagnostic Tests for Typhoid Diagnosis and Assessment of Febrile Illness Outbreaks in Fiji

Aneley Getahun Strobel*, Stephanie Airs, Cattram Nguyen, Taina Rokobuli Vadei, Silivia Matanitobua, Mike Kama, Conall H. Watson, John A. Crump, Edward Kim Mulholland, Richard A. Strugnell, Christopher M. Parry

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Typhoid is an endemic in Fiji with increases observed since the early 2000s and frequent outbreaks reported. We assessed the diagnostic accuracy of currently available typhoid rapid diagnostic tests (RDTs) (TUBEX, Typhidot Rapid, and Test-It assay) to establish their performance against blood culture in Fiji and to examine their suitability for rapid typhoid outbreak identification. The performance of RDTs was assessed in the public health reference laboratory in Suva, Fiji, according to the manufacturers' instructions. A simulation was used to examine the potential use of RDTs for attribution of a febrile illness outbreak to typhoid. For the diagnostic evaluation, 179 patients were included; 49 had blood culture-confirmed typhoid, 76 had fever as a result of non-typhoid etiologies, and 54 were age-matched community controls. The median (interquartile range) age was 29 (20-46) years. Of the participants, 92 (51.4%) were male and 131 (73.2%) were indigenous Fijians. The sensitivities of the tests were 77.6% for TUBEX, 75.5% for Typhidot Rapid, and 57.1% for Test-It assay. The Test-It assay had the highest specificity of 93.4%, followed by Typhidot Rapid 85.5% and TUBEX 60.5%. Typhidot Rapid had the best performance in the simulation for attribution of a febrile illness outbreak to typhoid. Typhoid RDTs performed suboptimally for individual patient diagnosis due to low sensitivity and variable specificity. We demonstrate that RDTs could be useful in the field for rapid attribution of febrile illness outbreaks to typhoid. Typhidot Rapid had the best combination of sensitivity, specificity, positive and negative predictive values, cost, and ease of use for this purpose.

Original languageEnglish
Pages (from-to)543-549
Number of pages7
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume106
Issue number2
DOIs
Publication statusPublished - Feb 2022
Externally publishedYes

Bibliographical note

Funding Information:
Financial support: This work was supported by Coalition against Typhoid through the Bill & Melinda Gates Foundation (grant number OPP1017518) and the Ministry of Education, Culture, Sports, Science, and Technology (MEXT), Government of Japan.

Publisher Copyright:
© 2022 American Society of Tropical Medicine and Hygiene. All rights reserved.

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