ARF mediates recruitment of Ptdlns-4-OH kinase-β and stimulates synthesis of Ptdlns(4,5)P2 on the Golgi complex

Anna Godi; Paolo Pertile; Rachel Meyers; Pierfrancesco Marra; Giuseppe Di Tullio; Cristiano Iurisci; Alberto Luini; Daniela Corda; Maria Antonietta De Matteis

doi:10.1038/12993

ARF mediates recruitment of Ptdlns-4-OH kinase-β and stimulates synthesis of Ptdlns(4,5)P₂ on the Golgi complex

Anna Godi^*, Paolo Pertile, Rachel Meyers, Pierfrancesco Marra, Giuseppe Di Tullio, Cristiano Iurisci, Alberto Luini, Daniela Corda, Maria Antonietta De Matteis

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

470 Citations (Scopus)

Abstract

The small GTPase ADP-ribosylation factor (ARF) regulates the structure and function of the Golgi complex through mechanisms that are understood only in part, and which include an ability to control the assembly of coat complexes and phospholipase D (PLD). Here we describe a new property of ARF, the ability to recruit phosphatidylinositol-4-OH kinase-β and a still unidentified phosphatidylinositol-4-phosphate-5-OH kinase to the Golgi complex, resulting in a potent stimulation of synthesis of phosphatidylinositol-4-phosphate and phosphatidylinositol-4,5-bisphosphate; this ability is independent of its activities on coat proteins and PLD. Phosphatidylinositol-4-OH kinase-β is required for the structural integrity of the Golgi complex: transfection of a dominant-negative mutant of the kinase markedly alters the organization of the organelle.

Original language	English
Pages (from-to)	280-287
Number of pages	8
Journal	Nature Cell Biology
Volume	1
Issue number	5
DOIs	https://doi.org/10.1038/12993
Publication status	Published - Sept 1999
Externally published	Yes

Bibliographical note

Funding Information:
ACKNOWLEDGEMENTS We thank C. Walch-Solimena and P. Novick for sharing unpublished results; J. Backer, R. Kahn, P. Hauri and F. Wieland for antibodies; J. Moss for the Drosophila ARFIII complementary DNA; M. Falasca for discussions; and C.P. Berrie for critical reading of the manuscript. This work was supported in part by grants from Telethon (E732), the Human Frontier Science Program (to M.A.D.M.), the Italian National Research Council (97.01300. PF49 and 97.01305.PF49) and the Italian Association for Cancer Research. A.G. and P.P. received fellowships from the Centro di Formazione e Studi per il Mezzogiorno (FORMEZ) and Banca di Roma, respectively. Correspondence and requests for materials should be addressed to M.A.D.M.

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title = "ARF mediates recruitment of Ptdlns-4-OH kinase-β and stimulates synthesis of Ptdlns(4,5)P2 on the Golgi complex",

abstract = "The small GTPase ADP-ribosylation factor (ARF) regulates the structure and function of the Golgi complex through mechanisms that are understood only in part, and which include an ability to control the assembly of coat complexes and phospholipase D (PLD). Here we describe a new property of ARF, the ability to recruit phosphatidylinositol-4-OH kinase-β and a still unidentified phosphatidylinositol-4-phosphate-5-OH kinase to the Golgi complex, resulting in a potent stimulation of synthesis of phosphatidylinositol-4-phosphate and phosphatidylinositol-4,5-bisphosphate; this ability is independent of its activities on coat proteins and PLD. Phosphatidylinositol-4-OH kinase-β is required for the structural integrity of the Golgi complex: transfection of a dominant-negative mutant of the kinase markedly alters the organization of the organelle.",

author = "Anna Godi and Paolo Pertile and Rachel Meyers and Pierfrancesco Marra and {Di Tullio}, Giuseppe and Cristiano Iurisci and Alberto Luini and Daniela Corda and {De Matteis}, {Maria Antonietta}",

note = "Funding Information: ACKNOWLEDGEMENTS We thank C. Walch-Solimena and P. Novick for sharing unpublished results; J. Backer, R. Kahn, P. Hauri and F. Wieland for antibodies; J. Moss for the Drosophila ARFIII complementary DNA; M. Falasca for discussions; and C.P. Berrie for critical reading of the manuscript. This work was supported in part by grants from Telethon (E732), the Human Frontier Science Program (to M.A.D.M.), the Italian National Research Council (97.01300. PF49 and 97.01305.PF49) and the Italian Association for Cancer Research. A.G. and P.P. received fellowships from the Centro di Formazione e Studi per il Mezzogiorno (FORMEZ) and Banca di Roma, respectively. Correspondence and requests for materials should be addressed to M.A.D.M.",

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AU - Godi, Anna

AU - Pertile, Paolo

AU - Meyers, Rachel

AU - Marra, Pierfrancesco

AU - Di Tullio, Giuseppe

AU - Iurisci, Cristiano

AU - Luini, Alberto

AU - Corda, Daniela

AU - De Matteis, Maria Antonietta

N1 - Funding Information: ACKNOWLEDGEMENTS We thank C. Walch-Solimena and P. Novick for sharing unpublished results; J. Backer, R. Kahn, P. Hauri and F. Wieland for antibodies; J. Moss for the Drosophila ARFIII complementary DNA; M. Falasca for discussions; and C.P. Berrie for critical reading of the manuscript. This work was supported in part by grants from Telethon (E732), the Human Frontier Science Program (to M.A.D.M.), the Italian National Research Council (97.01300. PF49 and 97.01305.PF49) and the Italian Association for Cancer Research. A.G. and P.P. received fellowships from the Centro di Formazione e Studi per il Mezzogiorno (FORMEZ) and Banca di Roma, respectively. Correspondence and requests for materials should be addressed to M.A.D.M.

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AB - The small GTPase ADP-ribosylation factor (ARF) regulates the structure and function of the Golgi complex through mechanisms that are understood only in part, and which include an ability to control the assembly of coat complexes and phospholipase D (PLD). Here we describe a new property of ARF, the ability to recruit phosphatidylinositol-4-OH kinase-β and a still unidentified phosphatidylinositol-4-phosphate-5-OH kinase to the Golgi complex, resulting in a potent stimulation of synthesis of phosphatidylinositol-4-phosphate and phosphatidylinositol-4,5-bisphosphate; this ability is independent of its activities on coat proteins and PLD. Phosphatidylinositol-4-OH kinase-β is required for the structural integrity of the Golgi complex: transfection of a dominant-negative mutant of the kinase markedly alters the organization of the organelle.

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ARF mediates recruitment of Ptdlns-4-OH kinase-β and stimulates synthesis of Ptdlns(4,5)P₂ on the Golgi complex

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