Application of polysaccharide microarray technology for the serodiagnosis of Burkholderia pseudomallei infection (melioidosis) in humans

Narayanan Parthasarathy*, David DeShazer, Sharon J. Peacock, Vanaporn Wuthiekanun, Marilyn J. England, Sarah L. Norris, David M. Waag

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

Burkholderia pseudomallei is the causative agent of melioidosis, a bacterial infection endemic in tropical regions including southeast Asia and northern Australia. B. pseudomallei contains structurally unique polysaccharides (capsular polysaccharide and O-antigen saccharides of lipopolysaccharide). A polysaccharide microarray platform was developed by immobilizing these polysaccharides onto glass slides. Employing this microarray, we were able to demonstrate the presence of antibodies to these polysaccharide antigens in the sera of melioidosis patients, but not in serum from nonmelioidosis human subjects. The advantages of this polysaccharide microarray technology over the conventional tests for the serodiagnosis of melioidosis are discussed.

Original languageEnglish
Pages (from-to)32-40
Number of pages9
JournalJournal of Carbohydrate Chemistry
Volume27
Issue number1
DOIs
Publication statusPublished - Jan 2008
Externally publishedYes

Bibliographical note

Funding Information:
The research described herein was sponsored by the U.S. Army Medical Research and Materiel Command under projects 06-4-2P-004 and AP-06-035. The use of human serum samples was approved by Human Use Committee (FY 06-36, HP-06-36). Opinions, interpretations, conclusions, and recommendations are those of the authors and not endorsed by the U.S. Army. The support of staff at Sappasithiprasong Hospital, Ubon Ratchathani, and the Mahidol Oxford Tropical Medicine Research Unit, particularly Dr. Direk Lim-mathurotsakul and Dr. Allen Cheng, is gratefully acknowledged. SP is funded by a Wellcome Trust Career Development Fellowship in Clinical Tropical Medicine.

Keywords

  • Burkholderia pseudomallei
  • Melioidosis
  • Polysaccharide microarray
  • Serodiagnosis

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