Application of continuous culture for assessing antibiotic activity against Mycobacterium tuberculosis

Charlotte L. Hendon-Dunn; Saba Anwar; Christopher Burton; Joanna Bacon

doi:10.1007/978-1-4939-7638-6_6

Application of continuous culture for assessing antibiotic activity against Mycobacterium tuberculosis

Charlotte L. Hendon-Dunn, Saba Anwar, Christopher Burton, Joanna Bacon^*

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

Abstract

There is a proportion of the M. tuberculosis population that is refractory to the bactericidal action of antituberculosis antibiotics due to phenotypic tolerance. This tolerance can be impacted by environmental stimuli and the subsequent physiological state of the organism. It may be the result of preexisting populations of slow growing/non replicating bacteria that are protected from antibiotic action. It still remains unclear how the slow growth of M. tuberculosis contributes to antibiotic resistance and antibiotic tolerance. Here, we present a method for assessing the activity of antibiotics against M. tuberculosis using continuous culture, which is the only system that can be used to control bacterial growth rate and study the impact of slow or fast growth on the organism’s response to antibiotic exposure.

Original language	English
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	59-73
Number of pages	15
DOIs	https://doi.org/10.1007/978-1-4939-7638-6_6
Publication status	Published - 2018

Publication series

Name	Methods in Molecular Biology
Volume	1736
ISSN (Print)	1064-3745

Bibliographical note

Funding Information:
Funding was received from Department of Health Grant in Aid and the National Institute of Health Research. The views expressed in this publication are those of the authors and not necessarily those of Public Health England, the National Institute for Health Research, or the Department of Health. The research leading to these results also received funding from the Innovative Medicines Initiative Joint Undertaking under grant agreement n°115337, resources of which are composed of financial contribution from the European Union’s Seventh Framework Programme (FP7/2007-2013) and EFPIA companies’ in-kind contribution. The authors acknowledge Kim Hatch and Jon Allnutt for the huge contribution they have made to the development of chemostat models for the growth of M. tuberculosis and application to the assessment of antibiotic activity and to Professor Philip Marsh for his guidance and constructive input.

Publisher Copyright:
© 2018, Springer Science+Business Media, LLC.

Keywords

Antibiotic resistance
Chemostat
Continuous culture
Mycobacteria

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/978-1-4939-7638-6_6

Cite this

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Application of continuous culture for assessing antibiotic activity against Mycobacterium tuberculosis

Abstract

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Bibliographical note

Keywords

UN SDGs

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