Application of carbohydrate microarray technology for the detection of Burkholderia pseudomallei, Bacillus anthracis and Francisella tularensis antibodies

N. Parthasarathy; R. Saksena; P. Kováč; D. DeShazer; S. J. Peacock; V. Wuthiekanun; H. S. Heine; A. M. Friedlander; C. K. Cote; S. L. Welkos; J. J. Adamovicz; S. Bavari; D. M. Waag

doi:10.1016/j.carres.2008.05.021

Application of carbohydrate microarray technology for the detection of Burkholderia pseudomallei, Bacillus anthracis and Francisella tularensis antibodies

N. Parthasarathy^*, R. Saksena, P. Kováč, D. DeShazer, S. J. Peacock, V. Wuthiekanun, H. S. Heine, A. M. Friedlander, C. K. Cote, S. L. Welkos, J. J. Adamovicz, S. Bavari, D. M. Waag

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

We developed a microarray platform by immobilizing bacterial 'signature' carbohydrates onto epoxide modified glass slides. The carbohydrate microarray platform was probed with sera from non-melioidosis and melioidosis (Burkholderia pseudomallei) individuals. The platform was also probed with sera from rabbits vaccinated with Bacillus anthracis spores and Francisella tularensis bacteria. By employing this microarray platform, we were able to detect and differentiate B. pseudomallei, B. anthracis and F. tularensis antibodies in infected patients, and infected or vaccinated animals. These antibodies were absent in the sera of nai{dotless}̈ve test subjects. The advantages of the carbohydrate microarray technology over the traditional indirect hemagglutination and microagglutination tests for the serodiagnosis of melioidosis and tularemia are discussed. Furthermore, this array is a multiplex carbohydrate microarray for the detection of all three biothreat bacterial infections including melioidosis, anthrax and tularemia with one, multivalent device. The implication is that this technology could be expanded to include a wide array of infectious and biothreat agents.

Original language	English
Pages (from-to)	2783-2788
Number of pages	6
Journal	Carbohydrate Research
Volume	343
Issue number	16
DOIs	https://doi.org/10.1016/j.carres.2008.05.021
Publication status	Published - 3 Nov 2008
Externally published	Yes

Bibliographical note

Funding Information:
The research described herein was sponsored by the US Army Medical Research and Materiel Command under projects 2.10018_06_RD-B, 2.10019_07_RD-B and 5.10023_08_RD-B. The authors thank Sarah L. Norris for statistical analysis. The authors also thank Dr. Sharan VedBrat (KamTek Inc., Gaithersburg, MD) for carbohydrate printing. The support of staff at Sappasithiprasong Hospital, Ubon Ratchathani, and the Mahidol Oxford Tropical Medicine Research Unit, particularly Dr. Direk Limmathurotsakul and Dr. Allen Chang, is gratefully acknowledged. S.P. is funded by a Welcome Trust Career Development fellowship in Clinical Tropical Medicine. The use of human serum samples was approved by the Human Use Committee (FY 06-36, HP-06-36). Research was conducted in compliance with the Animal Welfare Act and other federal statutes and regulations relating to animals and experiments involving animals and adheres to principles stated in the Guide for the Care and Use of Laboratory Animals, National Research Council, 1996. The facility where this research was conducted is fully accredited by the Association for Assessment and Accreditation of Laboratory Animal Care International. Opinions, interpretations, conclusions and recommendations are those of the authors and not endorsed by the US Army.

Keywords

Bacillus anthracis (anthrax)
Burkholderia pseudomallei (melioidosis)
Carbohydrate microarray
Francisella tularensis (tularemia)
Serum antibodies

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.carres.2008.05.021

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Parthasarathy, N., Saksena, R., Kováč, P., DeShazer, D., Peacock, S. J., Wuthiekanun, V., Heine, H. S., Friedlander, A. M., Cote, C. K., Welkos, S. L., Adamovicz, J. J., Bavari, S., & Waag, D. M. (2008). Application of carbohydrate microarray technology for the detection of Burkholderia pseudomallei, Bacillus anthracis and Francisella tularensis antibodies. Carbohydrate Research, 343(16), 2783-2788. https://doi.org/10.1016/j.carres.2008.05.021

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title = "Application of carbohydrate microarray technology for the detection of Burkholderia pseudomallei, Bacillus anthracis and Francisella tularensis antibodies",

abstract = "We developed a microarray platform by immobilizing bacterial 'signature' carbohydrates onto epoxide modified glass slides. The carbohydrate microarray platform was probed with sera from non-melioidosis and melioidosis (Burkholderia pseudomallei) individuals. The platform was also probed with sera from rabbits vaccinated with Bacillus anthracis spores and Francisella tularensis bacteria. By employing this microarray platform, we were able to detect and differentiate B. pseudomallei, B. anthracis and F. tularensis antibodies in infected patients, and infected or vaccinated animals. These antibodies were absent in the sera of nai{dotless{\"}}ve test subjects. The advantages of the carbohydrate microarray technology over the traditional indirect hemagglutination and microagglutination tests for the serodiagnosis of melioidosis and tularemia are discussed. Furthermore, this array is a multiplex carbohydrate microarray for the detection of all three biothreat bacterial infections including melioidosis, anthrax and tularemia with one, multivalent device. The implication is that this technology could be expanded to include a wide array of infectious and biothreat agents.",

keywords = "Bacillus anthracis (anthrax), Burkholderia pseudomallei (melioidosis), Carbohydrate microarray, Francisella tularensis (tularemia), Serum antibodies",

author = "N. Parthasarathy and R. Saksena and P. Kov{\'a}{\v c} and D. DeShazer and Peacock, {S. J.} and V. Wuthiekanun and Heine, {H. S.} and Friedlander, {A. M.} and Cote, {C. K.} and Welkos, {S. L.} and Adamovicz, {J. J.} and S. Bavari and Waag, {D. M.}",

note = "Funding Information: The research described herein was sponsored by the US Army Medical Research and Materiel Command under projects 2.10018_06_RD-B, 2.10019_07_RD-B and 5.10023_08_RD-B. The authors thank Sarah L. Norris for statistical analysis. The authors also thank Dr. Sharan VedBrat (KamTek Inc., Gaithersburg, MD) for carbohydrate printing. The support of staff at Sappasithiprasong Hospital, Ubon Ratchathani, and the Mahidol Oxford Tropical Medicine Research Unit, particularly Dr. Direk Limmathurotsakul and Dr. Allen Chang, is gratefully acknowledged. S.P. is funded by a Welcome Trust Career Development fellowship in Clinical Tropical Medicine. The use of human serum samples was approved by the Human Use Committee (FY 06-36, HP-06-36). Research was conducted in compliance with the Animal Welfare Act and other federal statutes and regulations relating to animals and experiments involving animals and adheres to principles stated in the Guide for the Care and Use of Laboratory Animals, National Research Council, 1996. The facility where this research was conducted is fully accredited by the Association for Assessment and Accreditation of Laboratory Animal Care International. Opinions, interpretations, conclusions and recommendations are those of the authors and not endorsed by the US Army.",

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month = nov,

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doi = "10.1016/j.carres.2008.05.021",

language = "English",

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Parthasarathy, N, Saksena, R, Kováč, P, DeShazer, D, Peacock, SJ, Wuthiekanun, V, Heine, HS, Friedlander, AM, Cote, CK, Welkos, SL, Adamovicz, JJ, Bavari, S & Waag, DM 2008, 'Application of carbohydrate microarray technology for the detection of Burkholderia pseudomallei, Bacillus anthracis and Francisella tularensis antibodies', Carbohydrate Research, vol. 343, no. 16, pp. 2783-2788. https://doi.org/10.1016/j.carres.2008.05.021

TY - JOUR

T1 - Application of carbohydrate microarray technology for the detection of Burkholderia pseudomallei, Bacillus anthracis and Francisella tularensis antibodies

AU - Parthasarathy, N.

AU - Saksena, R.

AU - Kováč, P.

AU - DeShazer, D.

AU - Peacock, S. J.

AU - Wuthiekanun, V.

AU - Heine, H. S.

AU - Friedlander, A. M.

AU - Cote, C. K.

AU - Welkos, S. L.

AU - Adamovicz, J. J.

AU - Bavari, S.

AU - Waag, D. M.

N1 - Funding Information: The research described herein was sponsored by the US Army Medical Research and Materiel Command under projects 2.10018_06_RD-B, 2.10019_07_RD-B and 5.10023_08_RD-B. The authors thank Sarah L. Norris for statistical analysis. The authors also thank Dr. Sharan VedBrat (KamTek Inc., Gaithersburg, MD) for carbohydrate printing. The support of staff at Sappasithiprasong Hospital, Ubon Ratchathani, and the Mahidol Oxford Tropical Medicine Research Unit, particularly Dr. Direk Limmathurotsakul and Dr. Allen Chang, is gratefully acknowledged. S.P. is funded by a Welcome Trust Career Development fellowship in Clinical Tropical Medicine. The use of human serum samples was approved by the Human Use Committee (FY 06-36, HP-06-36). Research was conducted in compliance with the Animal Welfare Act and other federal statutes and regulations relating to animals and experiments involving animals and adheres to principles stated in the Guide for the Care and Use of Laboratory Animals, National Research Council, 1996. The facility where this research was conducted is fully accredited by the Association for Assessment and Accreditation of Laboratory Animal Care International. Opinions, interpretations, conclusions and recommendations are those of the authors and not endorsed by the US Army.

PY - 2008/11/3

Y1 - 2008/11/3

N2 - We developed a microarray platform by immobilizing bacterial 'signature' carbohydrates onto epoxide modified glass slides. The carbohydrate microarray platform was probed with sera from non-melioidosis and melioidosis (Burkholderia pseudomallei) individuals. The platform was also probed with sera from rabbits vaccinated with Bacillus anthracis spores and Francisella tularensis bacteria. By employing this microarray platform, we were able to detect and differentiate B. pseudomallei, B. anthracis and F. tularensis antibodies in infected patients, and infected or vaccinated animals. These antibodies were absent in the sera of nai{dotless}̈ve test subjects. The advantages of the carbohydrate microarray technology over the traditional indirect hemagglutination and microagglutination tests for the serodiagnosis of melioidosis and tularemia are discussed. Furthermore, this array is a multiplex carbohydrate microarray for the detection of all three biothreat bacterial infections including melioidosis, anthrax and tularemia with one, multivalent device. The implication is that this technology could be expanded to include a wide array of infectious and biothreat agents.

AB - We developed a microarray platform by immobilizing bacterial 'signature' carbohydrates onto epoxide modified glass slides. The carbohydrate microarray platform was probed with sera from non-melioidosis and melioidosis (Burkholderia pseudomallei) individuals. The platform was also probed with sera from rabbits vaccinated with Bacillus anthracis spores and Francisella tularensis bacteria. By employing this microarray platform, we were able to detect and differentiate B. pseudomallei, B. anthracis and F. tularensis antibodies in infected patients, and infected or vaccinated animals. These antibodies were absent in the sera of nai{dotless}̈ve test subjects. The advantages of the carbohydrate microarray technology over the traditional indirect hemagglutination and microagglutination tests for the serodiagnosis of melioidosis and tularemia are discussed. Furthermore, this array is a multiplex carbohydrate microarray for the detection of all three biothreat bacterial infections including melioidosis, anthrax and tularemia with one, multivalent device. The implication is that this technology could be expanded to include a wide array of infectious and biothreat agents.

KW - Bacillus anthracis (anthrax)

KW - Burkholderia pseudomallei (melioidosis)

KW - Carbohydrate microarray

KW - Francisella tularensis (tularemia)

KW - Serum antibodies

UR - http://www.scopus.com/inward/record.url?scp=53049105779&partnerID=8YFLogxK

U2 - 10.1016/j.carres.2008.05.021

DO - 10.1016/j.carres.2008.05.021

M3 - Article

C2 - 18558401

AN - SCOPUS:53049105779

SN - 0008-6215

VL - 343

SP - 2783

EP - 2788

JO - Carbohydrate Research

JF - Carbohydrate Research

IS - 16

ER -

Application of carbohydrate microarray technology for the detection of Burkholderia pseudomallei, Bacillus anthracis and Francisella tularensis antibodies

Abstract

Bibliographical note

Keywords

UN SDGs

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