Analysis of Diagnostic Findings from the European Mobile Laboratory in Guéckédou, Guinea, March 2014 Through March 2015

Romy Kerber; Ralf Krumkamp; Boubacar DIallo; Anna Jaeger; Martin Rudolf; Simone Lanini; Joseph Akoi Bore; Fara Raymond Koundouno; Beate Becker-Ziaja; Erna Fleischmann; Kilian Stoecker; Silvia Meschi; Stéphane Mély; Edmund N.C. Newman; Fabrizio Carletti; Jasmine Portmann; Misa Korva; Svenja Wolff; Peter Molkenthin; Zoltan Kis; Anne Kelterbaum; Anne Bocquin; Thomas Strecker; Alexandra Fizet; Concetta Castilletti; Gordian Schudt; Lisa Ottowell; Andreas Kurth; Barry Atkinson; Marlis Badusche; Angela Cannas; Elisa Pallasch; Andrew Bosworth; Constanze Yue; Bernadett Pályi; Heinz Ellerbrok; Claudia Kohl; Lisa Oestereich; Christopher H. Logue; Anja Lüdtke; Martin Richter; DIdier Ngabo; Benny Borremans; DIrk Becker; Sophie Gryseels; Saïd Abdellati; Tine Vermoesen; Eeva Kuisma; Annette Kraus; Britta Liedigk; Piet Maes; Ruth Thom; Sophie Duraffour; Sandra DIederich; Julia Hinzmann; Babak Afrough; Johanna Repits; Marc Mertens; Inês Vitoriano; Amadou Bah; Andreas Sachse; Jan Peter Boettcher; Stephanie Wurr; Sabrina Bockholt; Andreas Nitsche; Tatjana Avšič Aupanc; Marc Strasser; Giuseppe Ippolito; Stephan Becker; Herve Raoul; Miles Carroll; Hilde De Clerck; Michel Van Herp; Armand Sprecher; Lamine Koivogui; N'Faly Magassouba; Sakoba Keïta; Patrick Drury; Cèline Gurry; Pierre Formenty; Jürgen May; Martin Gabriel; Roman Wölfel; Stephan Günther; Antonino DI Caro

doi:10.1093/infdis/jiw269

Analysis of Diagnostic Findings from the European Mobile Laboratory in Guéckédou, Guinea, March 2014 Through March 2015

Romy Kerber, Ralf Krumkamp, Boubacar DIallo, Anna Jaeger, Martin Rudolf, Simone Lanini, Joseph Akoi Bore, Fara Raymond Koundouno, Beate Becker-Ziaja, Erna Fleischmann, Kilian Stoecker, Silvia Meschi, Stéphane Mély, Edmund N.C. Newman, Fabrizio Carletti, Jasmine Portmann, Misa Korva, Svenja Wolff, Peter Molkenthin, Zoltan KisAnne Kelterbaum, Anne Bocquin, Thomas Strecker, Alexandra Fizet, Concetta Castilletti, Gordian Schudt, Lisa Ottowell, Andreas Kurth, Barry Atkinson, Marlis Badusche, Angela Cannas, Elisa Pallasch, Andrew Bosworth, Constanze Yue, Bernadett Pályi, Heinz Ellerbrok, Claudia Kohl, Lisa Oestereich, Christopher H. Logue, Anja Lüdtke, Martin Richter, DIdier Ngabo, Benny Borremans, DIrk Becker, Sophie Gryseels, Saïd Abdellati, Tine Vermoesen, Eeva Kuisma, Annette Kraus, Britta Liedigk, Piet Maes, Ruth Thom, Sophie Duraffour, Sandra DIederich, Julia Hinzmann, Babak Afrough, Johanna Repits, Marc Mertens, Inês Vitoriano, Amadou Bah, Andreas Sachse, Jan Peter Boettcher, Stephanie Wurr, Sabrina Bockholt, Andreas Nitsche, Tatjana Avšič Aupanc, Marc Strasser, Giuseppe Ippolito, Stephan Becker, Herve Raoul, Miles Carroll, Hilde De Clerck, Michel Van Herp, Armand Sprecher, Lamine Koivogui, N'Faly Magassouba, Sakoba Keïta, Patrick Drury, Cèline Gurry, Pierre Formenty, Jürgen May, Martin Gabriel, Roman Wölfel, Stephan Günther^*, Antonino DI Caro

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

33 Citations (Scopus)

Abstract

Background. A unit of the European Mobile Laboratory (EMLab) consortium was deployed to the Ebola virus disease (EVD) treatment unit in Guéckédou, Guinea, from March 2014 through March 2015. Methods. The unit diagnosed EVD and malaria, using the RealStar Filovirus Screen reverse transcription-polymerase chain reaction (RT-PCR) kit and a malaria rapid diagnostic test, respectively. Results. The cleaned EMLab database comprised 4719 samples from 2741 cases of suspected EVD from Guinea. EVD was diagnosed in 1231 of 2178 hospitalized patients (57%) and in 281 of 563 who died in the community (50%). Children aged <15 years had the highest proportion of Ebola virus-malaria parasite coinfections. The case-fatality ratio was high in patients aged <5 years (80%) and those aged >74 years (90%) and low in patients aged 10-19 years (40%). On admission, RT-PCR analysis of blood specimens from patients who died in the hospital yielded a lower median cycle threshold (Ct) than analysis of blood specimens from survivors (18.1 vs 23.2). Individuals who died in the community had a median Ct of 21.5 for throat swabs. Multivariate logistic regression on 1047 data sets revealed that low Ct values, ages of <5 and ≥45 years, and, among children aged 5-14 years, malaria parasite coinfection were independent determinants of a poor EVD outcome. Conclusions. Virus load, age, and malaria parasite coinfection play a role in the outcome of EVD.

Original language	English
Pages (from-to)	S250-S257
Journal	Journal of Infectious Diseases
Volume	214
DOIs	https://doi.org/10.1093/infdis/jiw269
Publication status	Published - 15 Oct 2016

Bibliographical note

Publisher Copyright:
© 2016 The Author.

Keywords

Ebola virus disease
Epidemic
Filovirus
Guinea
Malaria
Mobile laboratory

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1093/infdis/jiw269

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Kerber, R., Krumkamp, R., DIallo, B., Jaeger, A., Rudolf, M., Lanini, S., Bore, J. A., Koundouno, F. R., Becker-Ziaja, B., Fleischmann, E., Stoecker, K., Meschi, S., Mély, S., Newman, E. N. C., Carletti, F., Portmann, J., Korva, M., Wolff, S., Molkenthin, P., ... DI Caro, A. (2016). Analysis of Diagnostic Findings from the European Mobile Laboratory in Guéckédou, Guinea, March 2014 Through March 2015. Journal of Infectious Diseases, 214, S250-S257. https://doi.org/10.1093/infdis/jiw269

@article{a94e0381dc144ba2ad4e496464b8c7fc,

title = "Analysis of Diagnostic Findings from the European Mobile Laboratory in Gu{\'e}ck{\'e}dou, Guinea, March 2014 Through March 2015",

abstract = "Background. A unit of the European Mobile Laboratory (EMLab) consortium was deployed to the Ebola virus disease (EVD) treatment unit in Gu{\'e}ck{\'e}dou, Guinea, from March 2014 through March 2015. Methods. The unit diagnosed EVD and malaria, using the RealStar Filovirus Screen reverse transcription-polymerase chain reaction (RT-PCR) kit and a malaria rapid diagnostic test, respectively. Results. The cleaned EMLab database comprised 4719 samples from 2741 cases of suspected EVD from Guinea. EVD was diagnosed in 1231 of 2178 hospitalized patients (57%) and in 281 of 563 who died in the community (50%). Children aged <15 years had the highest proportion of Ebola virus-malaria parasite coinfections. The case-fatality ratio was high in patients aged <5 years (80%) and those aged >74 years (90%) and low in patients aged 10-19 years (40%). On admission, RT-PCR analysis of blood specimens from patients who died in the hospital yielded a lower median cycle threshold (Ct) than analysis of blood specimens from survivors (18.1 vs 23.2). Individuals who died in the community had a median Ct of 21.5 for throat swabs. Multivariate logistic regression on 1047 data sets revealed that low Ct values, ages of <5 and ≥45 years, and, among children aged 5-14 years, malaria parasite coinfection were independent determinants of a poor EVD outcome. Conclusions. Virus load, age, and malaria parasite coinfection play a role in the outcome of EVD.",

keywords = "Ebola virus disease, Epidemic, Filovirus, Guinea, Malaria, Mobile laboratory",

author = "Romy Kerber and Ralf Krumkamp and Boubacar DIallo and Anna Jaeger and Martin Rudolf and Simone Lanini and Bore, {Joseph Akoi} and Koundouno, {Fara Raymond} and Beate Becker-Ziaja and Erna Fleischmann and Kilian Stoecker and Silvia Meschi and St{\'e}phane M{\'e}ly and Newman, {Edmund N.C.} and Fabrizio Carletti and Jasmine Portmann and Misa Korva and Svenja Wolff and Peter Molkenthin and Zoltan Kis and Anne Kelterbaum and Anne Bocquin and Thomas Strecker and Alexandra Fizet and Concetta Castilletti and Gordian Schudt and Lisa Ottowell and Andreas Kurth and Barry Atkinson and Marlis Badusche and Angela Cannas and Elisa Pallasch and Andrew Bosworth and Constanze Yue and Bernadett P{\'a}lyi and Heinz Ellerbrok and Claudia Kohl and Lisa Oestereich and Logue, {Christopher H.} and Anja L{\"u}dtke and Martin Richter and DIdier Ngabo and Benny Borremans and DIrk Becker and Sophie Gryseels and Sa{\"i}d Abdellati and Tine Vermoesen and Eeva Kuisma and Annette Kraus and Britta Liedigk and Piet Maes and Ruth Thom and Sophie Duraffour and Sandra DIederich and Julia Hinzmann and Babak Afrough and Johanna Repits and Marc Mertens and In{\^e}s Vitoriano and Amadou Bah and Andreas Sachse and Boettcher, {Jan Peter} and Stephanie Wurr and Sabrina Bockholt and Andreas Nitsche and Aupanc, {Tatjana Av{\v s}i{\v c}} and Marc Strasser and Giuseppe Ippolito and Stephan Becker and Herve Raoul and Miles Carroll and {De Clerck}, Hilde and {Van Herp}, Michel and Armand Sprecher and Lamine Koivogui and N'Faly Magassouba and Sakoba Ke{\"i}ta and Patrick Drury and C{\`e}line Gurry and Pierre Formenty and J{\"u}rgen May and Martin Gabriel and Roman W{\"o}lfel and Stephan G{\"u}nther and {DI Caro}, Antonino",

note = "Publisher Copyright: {\textcopyright} 2016 The Author.",

year = "2016",

month = oct,

day = "15",

doi = "10.1093/infdis/jiw269",

language = "English",

volume = "214",

pages = "S250--S257",

journal = "Journal of Infectious Diseases",

issn = "0022-1899",

publisher = "Oxford University Press",

}

Kerber, R, Krumkamp, R, DIallo, B, Jaeger, A, Rudolf, M, Lanini, S, Bore, JA, Koundouno, FR, Becker-Ziaja, B, Fleischmann, E, Stoecker, K, Meschi, S, Mély, S, Newman, ENC, Carletti, F, Portmann, J, Korva, M, Wolff, S, Molkenthin, P, Kis, Z, Kelterbaum, A, Bocquin, A, Strecker, T, Fizet, A, Castilletti, C, Schudt, G, Ottowell, L, Kurth, A, Atkinson, B, Badusche, M, Cannas, A, Pallasch, E, Bosworth, A, Yue, C, Pályi, B, Ellerbrok, H, Kohl, C, Oestereich, L, Logue, CH, Lüdtke, A, Richter, M, Ngabo, DI, Borremans, B, Becker, DI, Gryseels, S, Abdellati, S, Vermoesen, T, Kuisma, E, Kraus, A, Liedigk, B, Maes, P, Thom, R, Duraffour, S, DIederich, S, Hinzmann, J, Afrough, B, Repits, J, Mertens, M, Vitoriano, I, Bah, A, Sachse, A, Boettcher, JP, Wurr, S, Bockholt, S, Nitsche, A, Aupanc, TA, Strasser, M, Ippolito, G, Becker, S, Raoul, H, Carroll, M, De Clerck, H, Van Herp, M, Sprecher, A, Koivogui, L, Magassouba, NF, Keïta, S, Drury, P, Gurry, C, Formenty, P, May, J, Gabriel, M, Wölfel, R, Günther, S & DI Caro, A 2016, 'Analysis of Diagnostic Findings from the European Mobile Laboratory in Guéckédou, Guinea, March 2014 Through March 2015', Journal of Infectious Diseases, vol. 214, pp. S250-S257. https://doi.org/10.1093/infdis/jiw269

TY - JOUR

T1 - Analysis of Diagnostic Findings from the European Mobile Laboratory in Guéckédou, Guinea, March 2014 Through March 2015

AU - Kerber, Romy

AU - Krumkamp, Ralf

AU - DIallo, Boubacar

AU - Jaeger, Anna

AU - Rudolf, Martin

AU - Lanini, Simone

AU - Bore, Joseph Akoi

AU - Koundouno, Fara Raymond

AU - Becker-Ziaja, Beate

AU - Fleischmann, Erna

AU - Stoecker, Kilian

AU - Meschi, Silvia

AU - Mély, Stéphane

AU - Newman, Edmund N.C.

AU - Carletti, Fabrizio

AU - Portmann, Jasmine

AU - Korva, Misa

AU - Wolff, Svenja

AU - Molkenthin, Peter

AU - Kis, Zoltan

AU - Kelterbaum, Anne

AU - Bocquin, Anne

AU - Strecker, Thomas

AU - Fizet, Alexandra

AU - Castilletti, Concetta

AU - Schudt, Gordian

AU - Ottowell, Lisa

AU - Kurth, Andreas

AU - Atkinson, Barry

AU - Badusche, Marlis

AU - Cannas, Angela

AU - Pallasch, Elisa

AU - Bosworth, Andrew

AU - Yue, Constanze

AU - Pályi, Bernadett

AU - Ellerbrok, Heinz

AU - Kohl, Claudia

AU - Oestereich, Lisa

AU - Logue, Christopher H.

AU - Lüdtke, Anja

AU - Richter, Martin

AU - Ngabo, DIdier

AU - Borremans, Benny

AU - Becker, DIrk

AU - Gryseels, Sophie

AU - Abdellati, Saïd

AU - Vermoesen, Tine

AU - Kuisma, Eeva

AU - Kraus, Annette

AU - Liedigk, Britta

AU - Maes, Piet

AU - Thom, Ruth

AU - Duraffour, Sophie

AU - DIederich, Sandra

AU - Hinzmann, Julia

AU - Afrough, Babak

AU - Repits, Johanna

AU - Mertens, Marc

AU - Vitoriano, Inês

AU - Bah, Amadou

AU - Sachse, Andreas

AU - Boettcher, Jan Peter

AU - Wurr, Stephanie

AU - Bockholt, Sabrina

AU - Nitsche, Andreas

AU - Aupanc, Tatjana Avšič

AU - Strasser, Marc

AU - Ippolito, Giuseppe

AU - Becker, Stephan

AU - Raoul, Herve

AU - Carroll, Miles

AU - De Clerck, Hilde

AU - Van Herp, Michel

AU - Sprecher, Armand

AU - Koivogui, Lamine

AU - Magassouba, N'Faly

AU - Keïta, Sakoba

AU - Drury, Patrick

AU - Gurry, Cèline

AU - Formenty, Pierre

AU - May, Jürgen

AU - Gabriel, Martin

AU - Wölfel, Roman

AU - Günther, Stephan

AU - DI Caro, Antonino

PY - 2016/10/15

Y1 - 2016/10/15

N2 - Background. A unit of the European Mobile Laboratory (EMLab) consortium was deployed to the Ebola virus disease (EVD) treatment unit in Guéckédou, Guinea, from March 2014 through March 2015. Methods. The unit diagnosed EVD and malaria, using the RealStar Filovirus Screen reverse transcription-polymerase chain reaction (RT-PCR) kit and a malaria rapid diagnostic test, respectively. Results. The cleaned EMLab database comprised 4719 samples from 2741 cases of suspected EVD from Guinea. EVD was diagnosed in 1231 of 2178 hospitalized patients (57%) and in 281 of 563 who died in the community (50%). Children aged <15 years had the highest proportion of Ebola virus-malaria parasite coinfections. The case-fatality ratio was high in patients aged <5 years (80%) and those aged >74 years (90%) and low in patients aged 10-19 years (40%). On admission, RT-PCR analysis of blood specimens from patients who died in the hospital yielded a lower median cycle threshold (Ct) than analysis of blood specimens from survivors (18.1 vs 23.2). Individuals who died in the community had a median Ct of 21.5 for throat swabs. Multivariate logistic regression on 1047 data sets revealed that low Ct values, ages of <5 and ≥45 years, and, among children aged 5-14 years, malaria parasite coinfection were independent determinants of a poor EVD outcome. Conclusions. Virus load, age, and malaria parasite coinfection play a role in the outcome of EVD.

AB - Background. A unit of the European Mobile Laboratory (EMLab) consortium was deployed to the Ebola virus disease (EVD) treatment unit in Guéckédou, Guinea, from March 2014 through March 2015. Methods. The unit diagnosed EVD and malaria, using the RealStar Filovirus Screen reverse transcription-polymerase chain reaction (RT-PCR) kit and a malaria rapid diagnostic test, respectively. Results. The cleaned EMLab database comprised 4719 samples from 2741 cases of suspected EVD from Guinea. EVD was diagnosed in 1231 of 2178 hospitalized patients (57%) and in 281 of 563 who died in the community (50%). Children aged <15 years had the highest proportion of Ebola virus-malaria parasite coinfections. The case-fatality ratio was high in patients aged <5 years (80%) and those aged >74 years (90%) and low in patients aged 10-19 years (40%). On admission, RT-PCR analysis of blood specimens from patients who died in the hospital yielded a lower median cycle threshold (Ct) than analysis of blood specimens from survivors (18.1 vs 23.2). Individuals who died in the community had a median Ct of 21.5 for throat swabs. Multivariate logistic regression on 1047 data sets revealed that low Ct values, ages of <5 and ≥45 years, and, among children aged 5-14 years, malaria parasite coinfection were independent determinants of a poor EVD outcome. Conclusions. Virus load, age, and malaria parasite coinfection play a role in the outcome of EVD.

KW - Ebola virus disease

KW - Epidemic

KW - Filovirus

KW - Guinea

KW - Malaria

KW - Mobile laboratory

UR - http://www.scopus.com/inward/record.url?scp=84991013851&partnerID=8YFLogxK

U2 - 10.1093/infdis/jiw269

DO - 10.1093/infdis/jiw269

M3 - Article

C2 - 27638946

AN - SCOPUS:84991013851

SN - 0022-1899

VL - 214

SP - S250-S257

JO - Journal of Infectious Diseases

JF - Journal of Infectious Diseases

ER -

Analysis of Diagnostic Findings from the European Mobile Laboratory in Guéckédou, Guinea, March 2014 Through March 2015

Abstract

Bibliographical note

Keywords

UN SDGs

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