An innovative standard operation procedure for isolating GMP-Grade CD4+CD25+ T cells from non-mobilized leukapheresis

Wei Zhang; Suzanne M. Watt; David J. Roberts

doi:10.1007/978-1-4939-8938-6_6

An innovative standard operation procedure for isolating GMP-Grade CD4⁺CD25⁺ T cells from non-mobilized leukapheresis

Wei Zhang^*
, Suzanne M. Watt
, David J. Roberts

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

Abstract

This SOP describes a closed system for isolating GMP-grade CD4⁺CD25⁺ T cells from non-mobilized leukapheresis collections (nMLCs), independent of a clean room in a certified GMP premises, by using CliniMACS format GMP grade reagents (CD25-labeled magnetic beads with/without pre-depletion of CD8⁺ T cells and CD19⁺ B cells), a GMP grade-A laminar hood and CliniMACS cell processing system.

Original language	English
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	67-83
Number of pages	17
DOIs	https://doi.org/10.1007/978-1-4939-8938-6_6
Publication status	Published - 2019
Externally published	Yes

Publication series

Name	Methods in Molecular Biology
Volume	1899
ISSN (Print)	1064-3745

Bibliographical note

Publisher Copyright:
© 2019, Springer Science+Business Media, LLC, part of Springer Nature.

Keywords

CD25
CliniMACS
GMP
Leukapheresis collection
Magnetic beads

Access to Document

10.1007/978-1-4939-8938-6_6

Cite this

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abstract = "This SOP describes a closed system for isolating GMP-grade CD4+CD25+ T cells from non-mobilized leukapheresis collections (nMLCs), independent of a clean room in a certified GMP premises, by using CliniMACS format GMP grade reagents (CD25-labeled magnetic beads with/without pre-depletion of CD8+ T cells and CD19+ B cells), a GMP grade-A laminar hood and CliniMACS cell processing system.",

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