An improved protocol for the preparation of total genomic DNA from isolates of yeast and mould using Whatman FTA filter papers

Andrew M. Borman; Mark Fraser; Christopher J. Linton; Michael D. Palmer; Elizabeth M. Johnson

doi:10.1007/s11046-010-9284-7

An improved protocol for the preparation of total genomic DNA from isolates of yeast and mould using Whatman FTA filter papers

Andrew M. Borman, Mark Fraser, Christopher J. Linton, Michael D. Palmer, Elizabeth M. Johnson

NIS: Bristol Mycology Reference Laboratory General

Research output: Contribution to journal › Article › peer-review

26 Citations (Scopus)

Abstract

Here, we present a significantly improved version of our previously published method for the extraction of fungal genomic DNA from pure cultures using Whatman FTA^® filter paper matrix technology. This modified protocol is extremely rapid, significantly more cost effective than our original method, and importantly, substantially reduces the problem of potential cross-contamination between sequential filters when employing FTA technology.

Original language	English
Pages (from-to)	445-449
Number of pages	5
Journal	Mycopathologia
Volume	169
Issue number	6
DOIs	https://doi.org/10.1007/s11046-010-9284-7
Publication status	Published - Jun 2010

Keywords

DNA extraction
FTA technology
Fungal genomic DNA
Molecular identification
PCR

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10.1007/s11046-010-9284-7

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An improved protocol for the preparation of total genomic DNA from isolates of yeast and mould using Whatman FTA filter papers

Abstract

Keywords

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