AN IMPEDANCE BASED FAST ANTIMICROBIAL SUSCEPTIBILITY TEST FOR PHENOTYPIC PROFILING

R. H.G. Mingels; B. Martin; L. Bock; D. C. Spencer; M. Sutton; R. C. Read; H. Morgan

AN IMPEDANCE BASED FAST ANTIMICROBIAL SUSCEPTIBILITY TEST FOR PHENOTYPIC PROFILING

R. H.G. Mingels^*, B. Martin, L. Bock, D. C. Spencer, M. Sutton, R. C. Read, H. Morgan

^*Corresponding author for this work

Research & Development Institute Scientific Leaders

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

New methods are needed to rapidly assess the antibiotic susceptibility of bacteria to allow informed prescription. In this work we report a new impedance based antimicrobial susceptibility test that can deliver susceptibility profiles of bacteria within 1-2 hours. The system measures the single bacteria at high speed and discriminates between susceptible and resistant strains (Staphylococcus Aureus) from changes in the electrical phenotype after exposure to cefoxitin. Interestingly, we observe opposite phenotypic change (cell size relative to control) contrary to previous data. These specific changes are dependent on antibiotic concentration, strain and time. A clear distinction can be made based on the mean cell diameter, allowing discrimination of sensitive and resistant strains in a rapid fashion.

Original language	English
Title of host publication	MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Publisher	Chemical and Biological Microsystems Society
Pages	783-784
Number of pages	2
ISBN (Electronic)	9781733419031
Publication status	Published - 2021
Event	25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021 - Palm Springs, Virtual, United States Duration: 10 Oct 2021 → 14 Oct 2021

Publication series

Name	MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Conference

Conference	25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021
Country/Territory	United States
City	Palm Springs, Virtual
Period	10/10/21 → 14/10/21

Bibliographical note

Publisher Copyright:
© 2021 MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved.

Keywords

Antimicrobial resistance
antibiotics
cytometry
impedance

Cite this

Mingels, R. H. G., Martin, B., Bock, L., Spencer, D. C., Sutton, M., Read, R. C., & Morgan, H. (2021). AN IMPEDANCE BASED FAST ANTIMICROBIAL SUSCEPTIBILITY TEST FOR PHENOTYPIC PROFILING. In MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 783-784). (MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences). Chemical and Biological Microsystems Society.

Mingels, R. H.G. ; Martin, B. ; Bock, L. et al. / AN IMPEDANCE BASED FAST ANTIMICROBIAL SUSCEPTIBILITY TEST FOR PHENOTYPIC PROFILING. MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, 2021. pp. 783-784 (MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences).

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title = "AN IMPEDANCE BASED FAST ANTIMICROBIAL SUSCEPTIBILITY TEST FOR PHENOTYPIC PROFILING",

abstract = "New methods are needed to rapidly assess the antibiotic susceptibility of bacteria to allow informed prescription. In this work we report a new impedance based antimicrobial susceptibility test that can deliver susceptibility profiles of bacteria within 1-2 hours. The system measures the single bacteria at high speed and discriminates between susceptible and resistant strains (Staphylococcus Aureus) from changes in the electrical phenotype after exposure to cefoxitin. Interestingly, we observe opposite phenotypic change (cell size relative to control) contrary to previous data. These specific changes are dependent on antibiotic concentration, strain and time. A clear distinction can be made based on the mean cell diameter, allowing discrimination of sensitive and resistant strains in a rapid fashion.",

keywords = "Antimicrobial resistance, antibiotics, cytometry, impedance",

author = "Mingels, {R. H.G.} and B. Martin and L. Bock and Spencer, {D. C.} and M. Sutton and Read, {R. C.} and H. Morgan",

note = "Publisher Copyright: {\textcopyright} 2021 MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved.; 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021 ; Conference date: 10-10-2021 Through 14-10-2021",

year = "2021",

language = "English",

series = "MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences",

publisher = "Chemical and Biological Microsystems Society",

pages = "783--784",

booktitle = "MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences",

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Mingels, RHG, Martin, B, Bock, L, Spencer, DC, Sutton, M, Read, RC & Morgan, H 2021, AN IMPEDANCE BASED FAST ANTIMICROBIAL SUSCEPTIBILITY TEST FOR PHENOTYPIC PROFILING. in MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, Chemical and Biological Microsystems Society, pp. 783-784, 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021, Palm Springs, Virtual, United States, 10/10/21.

AN IMPEDANCE BASED FAST ANTIMICROBIAL SUSCEPTIBILITY TEST FOR PHENOTYPIC PROFILING. / Mingels, R. H.G.; Martin, B.; Bock, L. et al.
MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, 2021. p. 783-784 (MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - AN IMPEDANCE BASED FAST ANTIMICROBIAL SUSCEPTIBILITY TEST FOR PHENOTYPIC PROFILING

AU - Mingels, R. H.G.

AU - Martin, B.

AU - Bock, L.

AU - Spencer, D. C.

AU - Sutton, M.

AU - Read, R. C.

AU - Morgan, H.

PY - 2021

Y1 - 2021

N2 - New methods are needed to rapidly assess the antibiotic susceptibility of bacteria to allow informed prescription. In this work we report a new impedance based antimicrobial susceptibility test that can deliver susceptibility profiles of bacteria within 1-2 hours. The system measures the single bacteria at high speed and discriminates between susceptible and resistant strains (Staphylococcus Aureus) from changes in the electrical phenotype after exposure to cefoxitin. Interestingly, we observe opposite phenotypic change (cell size relative to control) contrary to previous data. These specific changes are dependent on antibiotic concentration, strain and time. A clear distinction can be made based on the mean cell diameter, allowing discrimination of sensitive and resistant strains in a rapid fashion.

AB - New methods are needed to rapidly assess the antibiotic susceptibility of bacteria to allow informed prescription. In this work we report a new impedance based antimicrobial susceptibility test that can deliver susceptibility profiles of bacteria within 1-2 hours. The system measures the single bacteria at high speed and discriminates between susceptible and resistant strains (Staphylococcus Aureus) from changes in the electrical phenotype after exposure to cefoxitin. Interestingly, we observe opposite phenotypic change (cell size relative to control) contrary to previous data. These specific changes are dependent on antibiotic concentration, strain and time. A clear distinction can be made based on the mean cell diameter, allowing discrimination of sensitive and resistant strains in a rapid fashion.

KW - Antimicrobial resistance

KW - antibiotics

KW - cytometry

KW - impedance

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T3 - MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences

SP - 783

EP - 784

BT - MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences

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Y2 - 10 October 2021 through 14 October 2021

ER -

Mingels RHG, Martin B, Bock L, Spencer DC, Sutton M, Read RC et al. AN IMPEDANCE BASED FAST ANTIMICROBIAL SUSCEPTIBILITY TEST FOR PHENOTYPIC PROFILING. In MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society. 2021. p. 783-784. (MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences).

AN IMPEDANCE BASED FAST ANTIMICROBIAL SUSCEPTIBILITY TEST FOR PHENOTYPIC PROFILING

Abstract

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Keywords

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