An enzyme immunoassay for detecting Cryptosporidium in faecal and environmental samples

P. A. Chapman; B. A. Rush; James McLauchlin

doi:10.1099/00222615-32-4-233

An enzyme immunoassay for detecting Cryptosporidium in faecal and environmental samples

P. A. Chapman, B. A. Rush, James McLauchlin

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Abstract

An enzyme immunoassay (EIA) with a monoclonal antibody to cryptosporidium oocysts was developed and evaluated for the examination of faecal and environmental samples. The EIA was as sensitive as microscopy for detecting Cryptosporidium but also produced some positive results which could not be confirmed by a modified Ziehl-Neelsen technique or by immunofluorescence microscopy. These specimens reacted also with heterologous antibodies in EIA, suggesting false positive results.

Original language	English
Pages (from-to)	233-237
Number of pages	5
Journal	Journal of Medical Microbiology
Volume	32
Issue number	4
DOIs	https://doi.org/10.1099/00222615-32-4-233
Publication status	Published - 1990

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title = "An enzyme immunoassay for detecting Cryptosporidium in faecal and environmental samples",

abstract = "An enzyme immunoassay (EIA) with a monoclonal antibody to cryptosporidium oocysts was developed and evaluated for the examination of faecal and environmental samples. The EIA was as sensitive as microscopy for detecting Cryptosporidium but also produced some positive results which could not be confirmed by a modified Ziehl-Neelsen technique or by immunofluorescence microscopy. These specimens reacted also with heterologous antibodies in EIA, suggesting false positive results.",

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AB - An enzyme immunoassay (EIA) with a monoclonal antibody to cryptosporidium oocysts was developed and evaluated for the examination of faecal and environmental samples. The EIA was as sensitive as microscopy for detecting Cryptosporidium but also produced some positive results which could not be confirmed by a modified Ziehl-Neelsen technique or by immunofluorescence microscopy. These specimens reacted also with heterologous antibodies in EIA, suggesting false positive results.

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An enzyme immunoassay for detecting Cryptosporidium in faecal and environmental samples

Abstract

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