An assessment of different DNA delivery systems for protection against respiratory syncytial virus infection in the murine model: Gene-gun delivery induces IgG in the lung

Julia A. Tree; Gary Bembridge; Sam Hou; Geraldine Taylor; Elizabeth Fashola-Stone; José Melero; Martin P. Cranage

doi:10.1016/j.vaccine.2003.11.069

An assessment of different DNA delivery systems for protection against respiratory syncytial virus infection in the murine model: Gene-gun delivery induces IgG in the lung

Julia A. Tree, Gary Bembridge, Sam Hou, Geraldine Taylor, Elizabeth Fashola-Stone, José Melero, Martin P. Cranage^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

16 Citations (Scopus)

Abstract

Immunization with plasmid DNA (pDNA) has the potential to overcome the difficulties of neonatal vaccination that may be required for protection against infection with respiratory syncytial virus (RSV); however, little is known about optimal delivery modalities. In this pilot study we compared mucosal delivery of pDNA encoding RSV F protein encapsulated in poly(DL-lactide-co- glycolide) with delivery of pDNA by gene-gun for the induction of immunity in mice. Intra-gastric or intra-nasal immunization with various doses of microparticles induced weak low levels of RSV-specific serum antibodies in a proportion of mice; in contrast, gene-gun vaccination led to protective immunity associated with a humoral response. Interestingly, RSV-specific antibody was detected in lung fragment cultures following intradermal vaccination with the gene-gun.

Original language	English
Pages (from-to)	2438-2443
Number of pages	6
Journal	Vaccine
Volume	22
Issue number	19
DOIs	https://doi.org/10.1016/j.vaccine.2003.11.069
Publication status	Published - 23 Jun 2004

Bibliographical note

Funding Information:
We are grateful to Lisa Hyland for advice regarding fragment cultures. We thank Peter Beverley for useful discussions. The work was supported by the UK Department of Health. The views expressed in the publication are those of the authors and not necessarily those of the Department of Health.

Keywords

DNA vaccine
Lung antibodies
Respiratory syncytial virus

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/j.vaccine.2003.11.069

Cite this

@article{23858ae00847452384603a109ce7bf7b,

title = "An assessment of different DNA delivery systems for protection against respiratory syncytial virus infection in the murine model: Gene-gun delivery induces IgG in the lung",

abstract = "Immunization with plasmid DNA (pDNA) has the potential to overcome the difficulties of neonatal vaccination that may be required for protection against infection with respiratory syncytial virus (RSV); however, little is known about optimal delivery modalities. In this pilot study we compared mucosal delivery of pDNA encoding RSV F protein encapsulated in poly(DL-lactide-co- glycolide) with delivery of pDNA by gene-gun for the induction of immunity in mice. Intra-gastric or intra-nasal immunization with various doses of microparticles induced weak low levels of RSV-specific serum antibodies in a proportion of mice; in contrast, gene-gun vaccination led to protective immunity associated with a humoral response. Interestingly, RSV-specific antibody was detected in lung fragment cultures following intradermal vaccination with the gene-gun.",

keywords = "DNA vaccine, Lung antibodies, Respiratory syncytial virus",

author = "Tree, {Julia A.} and Gary Bembridge and Sam Hou and Geraldine Taylor and Elizabeth Fashola-Stone and Jos{\'e} Melero and Cranage, {Martin P.}",

note = "Funding Information: We are grateful to Lisa Hyland for advice regarding fragment cultures. We thank Peter Beverley for useful discussions. The work was supported by the UK Department of Health. The views expressed in the publication are those of the authors and not necessarily those of the Department of Health.",

year = "2004",

month = jun,

day = "23",

doi = "10.1016/j.vaccine.2003.11.069",

language = "English",

volume = "22",

pages = "2438--2443",

journal = "Vaccine",

issn = "0264-410X",

publisher = "Elsevier Ltd.",

number = "19",

}

TY - JOUR

T1 - An assessment of different DNA delivery systems for protection against respiratory syncytial virus infection in the murine model

T2 - Gene-gun delivery induces IgG in the lung

AU - Tree, Julia A.

AU - Bembridge, Gary

AU - Hou, Sam

AU - Taylor, Geraldine

AU - Fashola-Stone, Elizabeth

AU - Melero, José

AU - Cranage, Martin P.

N1 - Funding Information: We are grateful to Lisa Hyland for advice regarding fragment cultures. We thank Peter Beverley for useful discussions. The work was supported by the UK Department of Health. The views expressed in the publication are those of the authors and not necessarily those of the Department of Health.

PY - 2004/6/23

Y1 - 2004/6/23

N2 - Immunization with plasmid DNA (pDNA) has the potential to overcome the difficulties of neonatal vaccination that may be required for protection against infection with respiratory syncytial virus (RSV); however, little is known about optimal delivery modalities. In this pilot study we compared mucosal delivery of pDNA encoding RSV F protein encapsulated in poly(DL-lactide-co- glycolide) with delivery of pDNA by gene-gun for the induction of immunity in mice. Intra-gastric or intra-nasal immunization with various doses of microparticles induced weak low levels of RSV-specific serum antibodies in a proportion of mice; in contrast, gene-gun vaccination led to protective immunity associated with a humoral response. Interestingly, RSV-specific antibody was detected in lung fragment cultures following intradermal vaccination with the gene-gun.

AB - Immunization with plasmid DNA (pDNA) has the potential to overcome the difficulties of neonatal vaccination that may be required for protection against infection with respiratory syncytial virus (RSV); however, little is known about optimal delivery modalities. In this pilot study we compared mucosal delivery of pDNA encoding RSV F protein encapsulated in poly(DL-lactide-co- glycolide) with delivery of pDNA by gene-gun for the induction of immunity in mice. Intra-gastric or intra-nasal immunization with various doses of microparticles induced weak low levels of RSV-specific serum antibodies in a proportion of mice; in contrast, gene-gun vaccination led to protective immunity associated with a humoral response. Interestingly, RSV-specific antibody was detected in lung fragment cultures following intradermal vaccination with the gene-gun.

KW - DNA vaccine

KW - Lung antibodies

KW - Respiratory syncytial virus

UR - http://www.scopus.com/inward/record.url?scp=2942625680&partnerID=8YFLogxK

U2 - 10.1016/j.vaccine.2003.11.069

DO - 10.1016/j.vaccine.2003.11.069

M3 - Article

C2 - 15193407

AN - SCOPUS:2942625680

SN - 0264-410X

VL - 22

SP - 2438

EP - 2443

JO - Vaccine

JF - Vaccine

IS - 19

ER -

An assessment of different DNA delivery systems for protection against respiratory syncytial virus infection in the murine model: Gene-gun delivery induces IgG in the lung

Abstract

Bibliographical note

Keywords

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this