Abstract
AFLP analysis using four selective primers was performed on a set of 33 Listeria monocytogenes including strains from patients and foods implicated in outbreaks, human sporadic cases or foods. Strains were tested belonging to serovars 1/2a, 1/2b, 1/2c, 3b, and 4b. Using one of the primers, the AFLP technique generated 20 different sized DNA fragments. The 33 cultures segregated into 14 different patterns, each comprising 7-12 different fragments. Although the method was not sufficiently discriminatory for epidemiological typing, AFLP analysis reconfirmed the observation that L. monocytogenes comprises two major genetic groups: group 1 includes strains of serovars 1/2a and 1/2c, while group 2 serovars 1/2b, 3b and 4b.
| Original language | English |
|---|---|
| Pages (from-to) | 132-136 |
| Number of pages | 5 |
| Journal | Systematic and Applied Microbiology |
| Volume | 23 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2000 |
Bibliographical note
Funding Information:One of us (GR) was partially funded by an individual mobility grant from the Consiglio Nazionale delle Ricerche, Rome, Italy. The technical expertise of, and helpful discussion with, Dr JR Gibson and MD Hampton (Laboratory of Enteric Pathogens) and statistical advice from C Graham (PHLS Statistics Unit) is gratefully acknowledged. The helpful discussion and critical comments from Professor GM Grasso (University of Molise, Campobasso, Italy) is also acknowledged.
Keywords
- Amplified fragment length polymorphism (AFLP)
- Listeria monocytogenes