Amplified fragment length polymorphism (AFLP) analysis of Clostridium perfringens for epidemiological typing

James McLauchlin*, G. Ripabelli, M. M. Brett, John Threlfall

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

59 Citations (Scopus)

Abstract

Thirty-five Clostridium perfringens isolates from patients and foods implicated in seven outbreaks of suspected Cl. perfringens food poisoning together with five unrelated incidents were analysed by serotyping and amplified fragment length polymorphism (AFLP). Despite minor band differences, AFLP was found to be highly reproducible and 16 different profiles (each unique to the 12 incidents) were recognised. The results from both serotyping and AFLP analysis identified exactly the same groups of related cultures. It is concluded that AFLP can provide a rapid, sensitive and reproducible method for the typing of Cl. perfringens for outbreak investigation. Copyright (C) 2000 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)21-28
Number of pages8
JournalInternational Journal of Food Microbiology
Volume56
Issue number1
DOIs
Publication statusPublished - 25 May 2000

Bibliographical note

Funding Information:
One of us (G.R.) was partially funded by a short-term mobility grant from the Consiglio Nazionale delle Ricerche, Rome, Italy. The technical expertise and helpful discussion from Dr J.R. Gibson, M.D. Hampton and S. Sweeney of the PHLS Division of Gastrointestinal Infections is gratefully acknowledged. The helpful discussion and critical comments from Professor G.M. Grasso (University of Molise, Campobasso, Italy) is also acknowledged.

Keywords

  • AFLP
  • Clostridium perfringens
  • Epidemiological typing

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