Accuracy of enzyme-linked immunosorbent assay using crude and purified antigens for serodiagnosis of melioidosis

Narisara Chantratita; Vanaporn Wuthiekanun; Aunchalee Thanwisai; Direk Limmathurotsakul; Allen C. Cheng; Wirongrong Chierakul; Nicholas P.J. Day; Sharon J. Peacock

doi:10.1128/CVI.00289-06

Accuracy of enzyme-linked immunosorbent assay using crude and purified antigens for serodiagnosis of melioidosis

Narisara Chantratita^*
, Vanaporn Wuthiekanun
, Aunchalee Thanwisai
, Direk Limmathurotsakul
, Allen C. Cheng
, Wirongrong Chierakul
, Nicholas P.J. Day
, Sharon J. Peacock

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

51 Citations (Scopus)

Abstract

Five enzyme-linked immunosorbent assays developed to detect antibodies to different Burkholderia pseudomallei antigen preparations were evaluated as diagnostic tests for melioidosis in northeast Thailand. The highest diagnostic indices were observed for an affinity-purified antigen (sensitivity, 82%; specificity, 72%) and crude B. pseudomallei antigen (sensitivity, 81%; specificity, 70%), an improvement over the indirect hemagglutination assay (sensitivity, 73%; specificity, 64%).

Original language	English
Pages (from-to)	110-113
Number of pages	4
Journal	Clinical and Vaccine Immunology
Volume	14
Issue number	1
DOIs	https://doi.org/10.1128/CVI.00289-06
Publication status	Published - Jan 2007
Externally published	Yes

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title = "Accuracy of enzyme-linked immunosorbent assay using crude and purified antigens for serodiagnosis of melioidosis",

abstract = "Five enzyme-linked immunosorbent assays developed to detect antibodies to different Burkholderia pseudomallei antigen preparations were evaluated as diagnostic tests for melioidosis in northeast Thailand. The highest diagnostic indices were observed for an affinity-purified antigen (sensitivity, 82\%; specificity, 72\%) and crude B. pseudomallei antigen (sensitivity, 81\%; specificity, 70\%), an improvement over the indirect hemagglutination assay (sensitivity, 73\%; specificity, 64\%).",

author = "Narisara Chantratita and Vanaporn Wuthiekanun and Aunchalee Thanwisai and Direk Limmathurotsakul and Cheng, \{Allen C.\} and Wirongrong Chierakul and Day, \{Nicholas P.J.\} and Peacock, \{Sharon J.\}",

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T1 - Accuracy of enzyme-linked immunosorbent assay using crude and purified antigens for serodiagnosis of melioidosis

AU - Chantratita, Narisara

AU - Wuthiekanun, Vanaporn

AU - Thanwisai, Aunchalee

AU - Limmathurotsakul, Direk

AU - Cheng, Allen C.

AU - Chierakul, Wirongrong

AU - Day, Nicholas P.J.

AU - Peacock, Sharon J.

PY - 2007/1

Y1 - 2007/1

N2 - Five enzyme-linked immunosorbent assays developed to detect antibodies to different Burkholderia pseudomallei antigen preparations were evaluated as diagnostic tests for melioidosis in northeast Thailand. The highest diagnostic indices were observed for an affinity-purified antigen (sensitivity, 82%; specificity, 72%) and crude B. pseudomallei antigen (sensitivity, 81%; specificity, 70%), an improvement over the indirect hemagglutination assay (sensitivity, 73%; specificity, 64%).

AB - Five enzyme-linked immunosorbent assays developed to detect antibodies to different Burkholderia pseudomallei antigen preparations were evaluated as diagnostic tests for melioidosis in northeast Thailand. The highest diagnostic indices were observed for an affinity-purified antigen (sensitivity, 82%; specificity, 72%) and crude B. pseudomallei antigen (sensitivity, 81%; specificity, 70%), an improvement over the indirect hemagglutination assay (sensitivity, 73%; specificity, 64%).

UR - https://www.scopus.com/pages/publications/34247876275

U2 - 10.1128/CVI.00289-06

DO - 10.1128/CVI.00289-06

M3 - Article

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AN - SCOPUS:34247876275

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JF - Clinical and Vaccine Immunology

IS - 1

ER -

Accuracy of enzyme-linked immunosorbent assay using crude and purified antigens for serodiagnosis of melioidosis

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