Accuracy of enzyme-linked immunosorbent assay using crude and purified antigens for serodiagnosis of melioidosis

Narisara Chantratita*, Vanaporn Wuthiekanun, Aunchalee Thanwisai, Direk Limmathurotsakul, Allen C. Cheng, Wirongrong Chierakul, Nicholas P.J. Day, Sharon J. Peacock

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

45 Citations (Scopus)

Abstract

Five enzyme-linked immunosorbent assays developed to detect antibodies to different Burkholderia pseudomallei antigen preparations were evaluated as diagnostic tests for melioidosis in northeast Thailand. The highest diagnostic indices were observed for an affinity-purified antigen (sensitivity, 82%; specificity, 72%) and crude B. pseudomallei antigen (sensitivity, 81%; specificity, 70%), an improvement over the indirect hemagglutination assay (sensitivity, 73%; specificity, 64%).

Original languageEnglish
Pages (from-to)110-113
Number of pages4
JournalClinical and Vaccine Immunology
Volume14
Issue number1
DOIs
Publication statusPublished - Jan 2007
Externally publishedYes

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