A single radial haemolysis assay for antibody to H5 haemagglutinin

J. M. Wood; D. Melzack; R. W. Newman; D. L. Major; Maria Zambon; K. G. Nicholson; A. Podda

doi:10.1016/S0531-5131(01)00410-1

A single radial haemolysis assay for antibody to H5 haemagglutinin

J. M. Wood^*, D. Melzack, R. W. Newman, D. L. Major, Maria Zambon, K. G. Nicholson, A. Podda

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

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Abstract

Background: It has been recognised that the haemagglutination–inhibition (HI) test is not sufficiently sensitive to detect human antibody to H5N1 influenza virus and alternative serological tests are needed. A modified single radial haemolysis (SRH) test is described. Methods: Sera from H5N1 human cases in Hong Kong SAR and from H5 vaccine trials were tested by an H5 SRH test which included a virus adsorption step to remove cross-reactive antibody. Test specificity was examined using reference animal antisera. Results: The SRH test recognised antibody in a specific antiserum to A/Hong Kong/489/97 (H5N1) virus, yet rabbit antibody to an A (H10N7) avian virus and human antibody to A (H1N1) or A (H3N2) viruses did not react. H5 SRH antibody induced by human H5N1 virus infection and A/Duck/Singapore/97 (H5N3) vaccination, correlated with antibody detected by microneutralisation techniques. Conclusion: The modified SRH test offers an alternative serological technique for detecting antibody to H5 haemagglutinins, which does not depend on use of live pathogenic H5N1 virus.

Original language	English
Pages (from-to)	761-766
Number of pages	6
Journal	International Congress Series
Volume	1219
Issue number	C
DOIs	https://doi.org/10.1016/S0531-5131(01)00410-1
Publication status	Published - 1 Jan 2001

Keywords

H5 antibody
HA
Influenza
SRH

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/S0531-5131(01)00410-1

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title = "A single radial haemolysis assay for antibody to H5 haemagglutinin",

abstract = "Background: It has been recognised that the haemagglutination–inhibition (HI) test is not sufficiently sensitive to detect human antibody to H5N1 influenza virus and alternative serological tests are needed. A modified single radial haemolysis (SRH) test is described. Methods: Sera from H5N1 human cases in Hong Kong SAR and from H5 vaccine trials were tested by an H5 SRH test which included a virus adsorption step to remove cross-reactive antibody. Test specificity was examined using reference animal antisera. Results: The SRH test recognised antibody in a specific antiserum to A/Hong Kong/489/97 (H5N1) virus, yet rabbit antibody to an A (H10N7) avian virus and human antibody to A (H1N1) or A (H3N2) viruses did not react. H5 SRH antibody induced by human H5N1 virus infection and A/Duck/Singapore/97 (H5N3) vaccination, correlated with antibody detected by microneutralisation techniques. Conclusion: The modified SRH test offers an alternative serological technique for detecting antibody to H5 haemagglutinins, which does not depend on use of live pathogenic H5N1 virus.",

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AU - Zambon, Maria

AU - Nicholson, K. G.

AU - Podda, A.

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N2 - Background: It has been recognised that the haemagglutination–inhibition (HI) test is not sufficiently sensitive to detect human antibody to H5N1 influenza virus and alternative serological tests are needed. A modified single radial haemolysis (SRH) test is described. Methods: Sera from H5N1 human cases in Hong Kong SAR and from H5 vaccine trials were tested by an H5 SRH test which included a virus adsorption step to remove cross-reactive antibody. Test specificity was examined using reference animal antisera. Results: The SRH test recognised antibody in a specific antiserum to A/Hong Kong/489/97 (H5N1) virus, yet rabbit antibody to an A (H10N7) avian virus and human antibody to A (H1N1) or A (H3N2) viruses did not react. H5 SRH antibody induced by human H5N1 virus infection and A/Duck/Singapore/97 (H5N3) vaccination, correlated with antibody detected by microneutralisation techniques. Conclusion: The modified SRH test offers an alternative serological technique for detecting antibody to H5 haemagglutinins, which does not depend on use of live pathogenic H5N1 virus.

AB - Background: It has been recognised that the haemagglutination–inhibition (HI) test is not sufficiently sensitive to detect human antibody to H5N1 influenza virus and alternative serological tests are needed. A modified single radial haemolysis (SRH) test is described. Methods: Sera from H5N1 human cases in Hong Kong SAR and from H5 vaccine trials were tested by an H5 SRH test which included a virus adsorption step to remove cross-reactive antibody. Test specificity was examined using reference animal antisera. Results: The SRH test recognised antibody in a specific antiserum to A/Hong Kong/489/97 (H5N1) virus, yet rabbit antibody to an A (H10N7) avian virus and human antibody to A (H1N1) or A (H3N2) viruses did not react. H5 SRH antibody induced by human H5N1 virus infection and A/Duck/Singapore/97 (H5N3) vaccination, correlated with antibody detected by microneutralisation techniques. Conclusion: The modified SRH test offers an alternative serological technique for detecting antibody to H5 haemagglutinins, which does not depend on use of live pathogenic H5N1 virus.

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A single radial haemolysis assay for antibody to H5 haemagglutinin

Abstract

Keywords

UN SDGs

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