TY - JOUR
T1 - A sensitive chromogenic limulus amoebocyte lysate micro-assay for detection of endotoxin in human plasma and in water
AU - Piotrowicz, Boguslawa I.
AU - Edlin, S. E.
AU - McCartney, A. Christine
PY - 1985
Y1 - 1985
N2 - This chromogenic endotoxin assay involves a 45 min incubation of plasma extract or water with a mixture of Limulus amoebocyte lysate and the chromogenic substrate S-2423. Absorbance is measured in micro-titre plates. The assay allows the detection of 0.2 pg endotoxin per ml in water (0.002 Endotoxin Units/ml) and 1 pg endotoxin per ml in plasma (0.012 Endotoxin Units/ml). The intra-assay coefficient of variation is less than 5%.
AB - This chromogenic endotoxin assay involves a 45 min incubation of plasma extract or water with a mixture of Limulus amoebocyte lysate and the chromogenic substrate S-2423. Absorbance is measured in micro-titre plates. The assay allows the detection of 0.2 pg endotoxin per ml in water (0.002 Endotoxin Units/ml) and 1 pg endotoxin per ml in plasma (0.012 Endotoxin Units/ml). The intra-assay coefficient of variation is less than 5%.
UR - http://www.scopus.com/inward/record.url?scp=0022402158&partnerID=8YFLogxK
U2 - 10.1016/S0176-6724(85)80105-X
DO - 10.1016/S0176-6724(85)80105-X
M3 - Article
C2 - 4060918
AN - SCOPUS:0022402158
SN - 0176-6724
VL - 260
SP - 108
EP - 112
JO - Zentralblatt fur Bakteriologie Mikrobiologie und Hygiene - Abt. 1 Orig. A
JF - Zentralblatt fur Bakteriologie Mikrobiologie und Hygiene - Abt. 1 Orig. A
IS - 1
ER -