A reverse genetics system of African horse sickness virus reveals existence of primary replication

Eiko Matsuo; Cristina Celma; Polly Roy

doi:10.1016/j.febslet.2010.06.030

A reverse genetics system of African horse sickness virus reveals existence of primary replication

Eiko Matsuo
, Cristina Celma
, Polly Roy^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

38 Citations (Scopus)

Abstract

African horse sickness virus (AHSV), a member of the orbivirus genus of the family Reoviridae, is an insect-vectored pathogen of horses of concern to the equine industry. Studies on AHSV replication and pathogenesis have been hampered by the lack of reverse genetics allowing targeted mutation of viral genomes. We demonstrate that AHSV single-stranded RNA synthesized in vitro (core transcripts) is infectious and that there are distinct primary and secondary stages of the replication cycle. Transfection with a mixture of core transcripts from two different serotypes or a mixture of core transcripts and a T7 derived transcript resulted in the recovery of reassortant viruses. Recovery of infectious ASHV from nucleic acid will benefit investigation of the virus and the generation of attenuated vaccines.

Original language	English
Pages (from-to)	3386-3391
Number of pages	6
Journal	FEBS Letters
Volume	584
Issue number	15
DOIs	https://doi.org/10.1016/j.febslet.2010.06.030
Publication status	Published - Aug 2010
Externally published	Yes

Bibliographical note

Funding Information:
We thank Maria McCrossan (LSHTM) for technical help with electron microscopy. This work was funded by BBSRC (UK) .

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

AHSV
Core transcript
Primary replication
Reverse genetic
T7 transcript

Access to Document

10.1016/j.febslet.2010.06.030

Cite this

@article{49bc75824725493dae7e6c85aa64d0f0,

title = "A reverse genetics system of African horse sickness virus reveals existence of primary replication",

abstract = "African horse sickness virus (AHSV), a member of the orbivirus genus of the family Reoviridae, is an insect-vectored pathogen of horses of concern to the equine industry. Studies on AHSV replication and pathogenesis have been hampered by the lack of reverse genetics allowing targeted mutation of viral genomes. We demonstrate that AHSV single-stranded RNA synthesized in vitro (core transcripts) is infectious and that there are distinct primary and secondary stages of the replication cycle. Transfection with a mixture of core transcripts from two different serotypes or a mixture of core transcripts and a T7 derived transcript resulted in the recovery of reassortant viruses. Recovery of infectious ASHV from nucleic acid will benefit investigation of the virus and the generation of attenuated vaccines.",

keywords = "AHSV, Core transcript, Primary replication, Reverse genetic, T7 transcript",

author = "Eiko Matsuo and Cristina Celma and Polly Roy",

note = "Funding Information: We thank Maria McCrossan (LSHTM) for technical help with electron microscopy. This work was funded by BBSRC (UK) .",

year = "2010",

month = aug,

doi = "10.1016/j.febslet.2010.06.030",

language = "English",

volume = "584",

pages = "3386--3391",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "John Wiley and Sons Inc.",

number = "15",

}

TY - JOUR

T1 - A reverse genetics system of African horse sickness virus reveals existence of primary replication

AU - Matsuo, Eiko

AU - Celma, Cristina

AU - Roy, Polly

N1 - Funding Information: We thank Maria McCrossan (LSHTM) for technical help with electron microscopy. This work was funded by BBSRC (UK) .

PY - 2010/8

Y1 - 2010/8

N2 - African horse sickness virus (AHSV), a member of the orbivirus genus of the family Reoviridae, is an insect-vectored pathogen of horses of concern to the equine industry. Studies on AHSV replication and pathogenesis have been hampered by the lack of reverse genetics allowing targeted mutation of viral genomes. We demonstrate that AHSV single-stranded RNA synthesized in vitro (core transcripts) is infectious and that there are distinct primary and secondary stages of the replication cycle. Transfection with a mixture of core transcripts from two different serotypes or a mixture of core transcripts and a T7 derived transcript resulted in the recovery of reassortant viruses. Recovery of infectious ASHV from nucleic acid will benefit investigation of the virus and the generation of attenuated vaccines.

AB - African horse sickness virus (AHSV), a member of the orbivirus genus of the family Reoviridae, is an insect-vectored pathogen of horses of concern to the equine industry. Studies on AHSV replication and pathogenesis have been hampered by the lack of reverse genetics allowing targeted mutation of viral genomes. We demonstrate that AHSV single-stranded RNA synthesized in vitro (core transcripts) is infectious and that there are distinct primary and secondary stages of the replication cycle. Transfection with a mixture of core transcripts from two different serotypes or a mixture of core transcripts and a T7 derived transcript resulted in the recovery of reassortant viruses. Recovery of infectious ASHV from nucleic acid will benefit investigation of the virus and the generation of attenuated vaccines.

KW - AHSV

KW - Core transcript

KW - Primary replication

KW - Reverse genetic

KW - T7 transcript

UR - https://www.scopus.com/pages/publications/77955271538

U2 - 10.1016/j.febslet.2010.06.030

DO - 10.1016/j.febslet.2010.06.030

M3 - Article

C2 - 20600010

AN - SCOPUS:77955271538

SN - 0014-5793

VL - 584

SP - 3386

EP - 3391

JO - FEBS Letters

JF - FEBS Letters

IS - 15

ER -

A reverse genetics system of African horse sickness virus reveals existence of primary replication

Abstract

Bibliographical note

UN SDGs

Keywords

Access to Document

Other files and links

Fingerprint

Cite this