A reverse genetics approach for recovery of recombinant influenza B viruses entirely from cDNA

David Jackson; Andrew Cadman; Thomas Zurcher; Wendy S. Barclay

doi:10.1128/JVI.76.22.11744-11747.2002

A reverse genetics approach for recovery of recombinant influenza B viruses entirely from cDNA

David Jackson, Andrew Cadman, Thomas Zurcher, Wendy S. Barclay^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

74 Citations (Scopus)

Abstract

The recovery of recombinant influenza A virus entirely from cDNA was recently described (9, 19). We adapted the technique for engineering influenza B virus and generated a mutant bearing an amino acid change E116G in the viral neuraminidase which was resistant in vitro to the neuraminidase inhibitor zanamivir. The method also facilitates rapid isolation of single-gene reassortants suitable as vaccine seeds and will aid further investigations of unique features of influenza B virus.

Original language	English
Pages (from-to)	11744-11747
Number of pages	4
Journal	Journal of Virology
Volume	76
Issue number	22
DOIs	https://doi.org/10.1128/JVI.76.22.11744-11747.2002
Publication status	Published - Nov 2002
Externally published	Yes

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10.1128/JVI.76.22.11744-11747.2002

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abstract = "The recovery of recombinant influenza A virus entirely from cDNA was recently described (9, 19). We adapted the technique for engineering influenza B virus and generated a mutant bearing an amino acid change E116G in the viral neuraminidase which was resistant in vitro to the neuraminidase inhibitor zanamivir. The method also facilitates rapid isolation of single-gene reassortants suitable as vaccine seeds and will aid further investigations of unique features of influenza B virus.",

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A reverse genetics approach for recovery of recombinant influenza B viruses entirely from cDNA

Abstract

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