A pathological study of Leishmania infantum natural infection in European rabbits (Oryctolagus cuniculus) and Iberian hares (Lepus granatensis)

María Victoria Ortega-García; Francisco Javier Salguero Bodes; Antonio Rodríguez-Bertos; Inmaculada Moreno; Nerea García; Teresa García-Seco; Gabriela Luz Torre; Lucas Domínguez; Mercedes Domínguez

doi:10.1111/tbed.13305

A pathological study of Leishmania infantum natural infection in European rabbits (Oryctolagus cuniculus) and Iberian hares (Lepus granatensis)

María Victoria Ortega-García, Francisco Javier Salguero Bodes^*, Antonio Rodríguez-Bertos, Inmaculada Moreno, Nerea García, Teresa García-Seco, Gabriela Luz Torre, Lucas Domínguez, Mercedes Domínguez

^*Corresponding author for this work

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Abstract

In this study, we describe the pathology of Leishmania infantum infection in naturally infected wild Leporidae and compare diagnosis of infection using histopathology, direct fluorescent antibody (DFA) assay, immunofluorescence antibody test (IFAT) and quantitative real-time PCR (qPCR). Tissues were analysed from 52 European rabbits (Oryctolagus cuniculus) and 7 Iberian hares (Lepus granatensis) from the Community of Madrid (Spain). Our results show that L. infantum infection is associated with only minimal histopathological lesions and that L. infantum amastigotes can be detected by DFA assay in all tissues types tested, including skin. These results were confirmed by qPCR on fresh frozen tissues in 13% of rabbits and 100% of hares. However, L. infantum DNA could not be detected by qPCR on paraffin-embedded tissue obtained by laser capture microdissection. Using the DFA assay to diagnose L. infantum, infection may provide further insights into this disease in wild animals and may allow the precise tissue localization of L. infantum, thereby guiding follow-up tests with more accurate qPCR.

Original language	English
Pages (from-to)	2474-2481
Number of pages	8
Journal	Transboundary and Emerging Diseases
Volume	66
Issue number	6
DOIs	https://doi.org/10.1111/tbed.13305
Publication status	Published - 1 Nov 2019

Bibliographical note

Funding Information:
This study was supported by the Ayuntamiento de Madrid of the Comunidad de Madrid [S2013/ABI-2747 (TAVS-CM)], by the Structural Funds of the European Union and by intramural funding from the University of Surrey.

Keywords

Leishmania infantum
diagnostic
histopathology
immunofluorescence
wild animals

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10.1111/tbed.13305

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Ortega-García, M. V., Salguero Bodes, F. J., Rodríguez-Bertos, A., Moreno, I., García, N., García-Seco, T., Luz Torre, G., Domínguez, L., & Domínguez, M. (2019). A pathological study of Leishmania infantum natural infection in European rabbits (Oryctolagus cuniculus) and Iberian hares (Lepus granatensis). Transboundary and Emerging Diseases, 66(6), 2474-2481. https://doi.org/10.1111/tbed.13305

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title = "A pathological study of Leishmania infantum natural infection in European rabbits (Oryctolagus cuniculus) and Iberian hares (Lepus granatensis)",

abstract = "In this study, we describe the pathology of Leishmania infantum infection in naturally infected wild Leporidae and compare diagnosis of infection using histopathology, direct fluorescent antibody (DFA) assay, immunofluorescence antibody test (IFAT) and quantitative real-time PCR (qPCR). Tissues were analysed from 52 European rabbits (Oryctolagus cuniculus) and 7 Iberian hares (Lepus granatensis) from the Community of Madrid (Spain). Our results show that L. infantum infection is associated with only minimal histopathological lesions and that L. infantum amastigotes can be detected by DFA assay in all tissues types tested, including skin. These results were confirmed by qPCR on fresh frozen tissues in 13% of rabbits and 100% of hares. However, L. infantum DNA could not be detected by qPCR on paraffin-embedded tissue obtained by laser capture microdissection. Using the DFA assay to diagnose L. infantum, infection may provide further insights into this disease in wild animals and may allow the precise tissue localization of L. infantum, thereby guiding follow-up tests with more accurate qPCR.",

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author = "Ortega-Garc{\'i}a, {Mar{\'i}a Victoria} and {Salguero Bodes}, {Francisco Javier} and Antonio Rodr{\'i}guez-Bertos and Inmaculada Moreno and Nerea Garc{\'i}a and Teresa Garc{\'i}a-Seco and {Luz Torre}, Gabriela and Lucas Dom{\'i}nguez and Mercedes Dom{\'i}nguez",

note = "Funding Information: This study was supported by the Ayuntamiento de Madrid of the Comunidad de Madrid [S2013/ABI-2747 (TAVS-CM)], by the Structural Funds of the European Union and by intramural funding from the University of Surrey.",

year = "2019",

month = nov,

day = "1",

doi = "10.1111/tbed.13305",

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Ortega-García, MV, Salguero Bodes, FJ, Rodríguez-Bertos, A, Moreno, I, García, N, García-Seco, T, Luz Torre, G, Domínguez, L & Domínguez, M 2019, 'A pathological study of Leishmania infantum natural infection in European rabbits (Oryctolagus cuniculus) and Iberian hares (Lepus granatensis)', Transboundary and Emerging Diseases, vol. 66, no. 6, pp. 2474-2481. https://doi.org/10.1111/tbed.13305

A pathological study of Leishmania infantum natural infection in European rabbits (Oryctolagus cuniculus) and Iberian hares (Lepus granatensis). / Ortega-García, María Victoria; Salguero Bodes, Francisco Javier; Rodríguez-Bertos, Antonio et al.
In: Transboundary and Emerging Diseases, Vol. 66, No. 6, 01.11.2019, p. 2474-2481.

Research output: Contribution to journal › Article › peer-review

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T1 - A pathological study of Leishmania infantum natural infection in European rabbits (Oryctolagus cuniculus) and Iberian hares (Lepus granatensis)

AU - Ortega-García, María Victoria

AU - Salguero Bodes, Francisco Javier

AU - Rodríguez-Bertos, Antonio

AU - Moreno, Inmaculada

AU - García, Nerea

AU - García-Seco, Teresa

AU - Luz Torre, Gabriela

AU - Domínguez, Lucas

AU - Domínguez, Mercedes

N1 - Funding Information: This study was supported by the Ayuntamiento de Madrid of the Comunidad de Madrid [S2013/ABI-2747 (TAVS-CM)], by the Structural Funds of the European Union and by intramural funding from the University of Surrey.

PY - 2019/11/1

Y1 - 2019/11/1

N2 - In this study, we describe the pathology of Leishmania infantum infection in naturally infected wild Leporidae and compare diagnosis of infection using histopathology, direct fluorescent antibody (DFA) assay, immunofluorescence antibody test (IFAT) and quantitative real-time PCR (qPCR). Tissues were analysed from 52 European rabbits (Oryctolagus cuniculus) and 7 Iberian hares (Lepus granatensis) from the Community of Madrid (Spain). Our results show that L. infantum infection is associated with only minimal histopathological lesions and that L. infantum amastigotes can be detected by DFA assay in all tissues types tested, including skin. These results were confirmed by qPCR on fresh frozen tissues in 13% of rabbits and 100% of hares. However, L. infantum DNA could not be detected by qPCR on paraffin-embedded tissue obtained by laser capture microdissection. Using the DFA assay to diagnose L. infantum, infection may provide further insights into this disease in wild animals and may allow the precise tissue localization of L. infantum, thereby guiding follow-up tests with more accurate qPCR.

AB - In this study, we describe the pathology of Leishmania infantum infection in naturally infected wild Leporidae and compare diagnosis of infection using histopathology, direct fluorescent antibody (DFA) assay, immunofluorescence antibody test (IFAT) and quantitative real-time PCR (qPCR). Tissues were analysed from 52 European rabbits (Oryctolagus cuniculus) and 7 Iberian hares (Lepus granatensis) from the Community of Madrid (Spain). Our results show that L. infantum infection is associated with only minimal histopathological lesions and that L. infantum amastigotes can be detected by DFA assay in all tissues types tested, including skin. These results were confirmed by qPCR on fresh frozen tissues in 13% of rabbits and 100% of hares. However, L. infantum DNA could not be detected by qPCR on paraffin-embedded tissue obtained by laser capture microdissection. Using the DFA assay to diagnose L. infantum, infection may provide further insights into this disease in wild animals and may allow the precise tissue localization of L. infantum, thereby guiding follow-up tests with more accurate qPCR.

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KW - diagnostic

KW - histopathology

KW - immunofluorescence

KW - wild animals

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DO - 10.1111/tbed.13305

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SN - 1865-1674

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JO - Transboundary and Emerging Diseases

JF - Transboundary and Emerging Diseases

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ER -

A pathological study of Leishmania infantum natural infection in European rabbits (Oryctolagus cuniculus) and Iberian hares (Lepus granatensis)

Abstract

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Keywords

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