A micromethod for endotoxin assay in human plasma using limulus amoebocyte lysate and a chromogenic substrate

B. I. Piotrowicz; I. Watt; S. Edlin; A. C. McCartney

doi:10.1007/BF02148661

A micromethod for endotoxin assay in human plasma using limulus amoebocyte lysate and a chromogenic substrate

B. I. Piotrowicz, I. Watt, S. Edlin, A. C. McCartney^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

A modified micromethod for endotoxin assay is reported which involved incubation of plasma extract with Limulus amoebocyte lysate for 30 min followed by incubation with chromogenie substrate S-2423 for another 8 min. Absorbance is measured in microtitre plates. The sensitivity is less than 5 pg/ml (0.058 Endotoxin Units/ml).

Original language	English
Pages (from-to)	52-54
Number of pages	3
Journal	European Journal of Clinical Microbiology
Volume	4
Issue number	1
DOIs	https://doi.org/10.1007/BF02148661
Publication status	Published - Feb 1985
Externally published	Yes

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title = "A micromethod for endotoxin assay in human plasma using limulus amoebocyte lysate and a chromogenic substrate",

abstract = "A modified micromethod for endotoxin assay is reported which involved incubation of plasma extract with Limulus amoebocyte lysate for 30 min followed by incubation with chromogenie substrate S-2423 for another 8 min. Absorbance is measured in microtitre plates. The sensitivity is less than 5 pg/ml (0.058 Endotoxin Units/ml).",

author = "Piotrowicz, {B. I.} and I. Watt and S. Edlin and McCartney, {A. C.}",

year = "1985",

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doi = "10.1007/BF02148661",

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journal = "European Journal of Clinical Microbiology",

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AU - Piotrowicz, B. I.

AU - Watt, I.

AU - Edlin, S.

AU - McCartney, A. C.

PY - 1985/2

Y1 - 1985/2

N2 - A modified micromethod for endotoxin assay is reported which involved incubation of plasma extract with Limulus amoebocyte lysate for 30 min followed by incubation with chromogenie substrate S-2423 for another 8 min. Absorbance is measured in microtitre plates. The sensitivity is less than 5 pg/ml (0.058 Endotoxin Units/ml).

AB - A modified micromethod for endotoxin assay is reported which involved incubation of plasma extract with Limulus amoebocyte lysate for 30 min followed by incubation with chromogenie substrate S-2423 for another 8 min. Absorbance is measured in microtitre plates. The sensitivity is less than 5 pg/ml (0.058 Endotoxin Units/ml).

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U2 - 10.1007/BF02148661

DO - 10.1007/BF02148661

M3 - Article

C2 - 3987679

AN - SCOPUS:0021801272

VL - 4

SP - 52

EP - 54

JO - European Journal of Clinical Microbiology

JF - European Journal of Clinical Microbiology

SN - 0722-2211

IS - 1

ER -

A micromethod for endotoxin assay in human plasma using limulus amoebocyte lysate and a chromogenic substrate

Abstract

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