A micromethod for endotoxin assay in human plasma using limulus amoebocyte lysate and a chromogenic substrate

B. I. Piotrowicz, I. Watt, S. Edlin, A. C. McCartney*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

A modified micromethod for endotoxin assay is reported which involved incubation of plasma extract with Limulus amoebocyte lysate for 30 min followed by incubation with chromogenie substrate S-2423 for another 8 min. Absorbance is measured in microtitre plates. The sensitivity is less than 5 pg/ml (0.058 Endotoxin Units/ml).

Original languageEnglish
Pages (from-to)52-54
Number of pages3
JournalEuropean Journal of Clinical Microbiology
Volume4
Issue number1
DOIs
Publication statusPublished - Feb 1985
Externally publishedYes

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