A method for increasing the surface details of resin embedded viruses using congo/crimean haemorrhagic fever virus as a model

D. S. Ellis; M. D. Smith; Graham Lloyd; E. T.W. Bowen; D. I.H. Simpson

doi:10.1016/0166-0934(84)90040-5

A method for increasing the surface details of resin embedded viruses using congo/crimean haemorrhagic fever virus as a model

D. S. Ellis^*, M. D. Smith, Graham Lloyd, E. T.W. Bowen, D. I.H. Simpson

^*Corresponding author for this work

Public Health England

Research output: Contribution to journal › Article › peer-review

Abstract

The surface details of resin embedded viruses can be greatly enhanced by the use of uranyl acetate block staining. The virions are embedded in agar immediately after fixation and block stained with 3% uranyl acetate in methanol during dehydration. This technique allows those particles outside their host cells to show clearly the spikes normally seen only by the separate use of negative staining.

Original language	English
Pages (from-to)	57-61
Number of pages	5
Journal	Journal of Virological Methods
Volume	8
Issue number	1-2
DOIs	https://doi.org/10.1016/0166-0934(84)90040-5
Publication status	Published - Feb 1984

Bibliographical note

Funding Information:
This work wass upportedb y a grantf rom theW ellcomeT rust. The authorsw isht o thank Springer-VerlagV, ienna, for permissiont o re-useF igs. 1, 2a nd 4 which had previouslya ppearedin Archiveso f Virology (Ellis et al., 1981).

Keywords

electron microscopy staining
virus visualisation

Access to Document

10.1016/0166-0934(84)90040-5

Cite this

@article{406db9a0a70a4c8190959f2ff712d078,

title = "A method for increasing the surface details of resin embedded viruses using congo/crimean haemorrhagic fever virus as a model",

abstract = "The surface details of resin embedded viruses can be greatly enhanced by the use of uranyl acetate block staining. The virions are embedded in agar immediately after fixation and block stained with 3% uranyl acetate in methanol during dehydration. This technique allows those particles outside their host cells to show clearly the spikes normally seen only by the separate use of negative staining.",

keywords = "electron microscopy staining, virus visualisation",

author = "Ellis, {D. S.} and Smith, {M. D.} and Graham Lloyd and Bowen, {E. T.W.} and Simpson, {D. I.H.}",

note = "Funding Information: This work wass upportedb y a grantf rom theW ellcomeT rust. The authorsw isht o thank Springer-VerlagV, ienna, for permissiont o re-useF igs. 1, 2a nd 4 which had previouslya ppearedin Archiveso f Virology (Ellis et al., 1981).",

year = "1984",

month = feb,

doi = "10.1016/0166-0934(84)90040-5",

language = "English",

volume = "8",

pages = "57--61",

journal = "Journal of Virological Methods",

issn = "0166-0934",

publisher = "Elsevier",

number = "1-2",

}

TY - JOUR

T1 - A method for increasing the surface details of resin embedded viruses using congo/crimean haemorrhagic fever virus as a model

AU - Ellis, D. S.

AU - Smith, M. D.

AU - Lloyd, Graham

AU - Bowen, E. T.W.

AU - Simpson, D. I.H.

N1 - Funding Information: This work wass upportedb y a grantf rom theW ellcomeT rust. The authorsw isht o thank Springer-VerlagV, ienna, for permissiont o re-useF igs. 1, 2a nd 4 which had previouslya ppearedin Archiveso f Virology (Ellis et al., 1981).

PY - 1984/2

Y1 - 1984/2

N2 - The surface details of resin embedded viruses can be greatly enhanced by the use of uranyl acetate block staining. The virions are embedded in agar immediately after fixation and block stained with 3% uranyl acetate in methanol during dehydration. This technique allows those particles outside their host cells to show clearly the spikes normally seen only by the separate use of negative staining.

AB - The surface details of resin embedded viruses can be greatly enhanced by the use of uranyl acetate block staining. The virions are embedded in agar immediately after fixation and block stained with 3% uranyl acetate in methanol during dehydration. This technique allows those particles outside their host cells to show clearly the spikes normally seen only by the separate use of negative staining.

KW - electron microscopy staining

KW - virus visualisation

UR - http://www.scopus.com/inward/record.url?scp=0021354559&partnerID=8YFLogxK

U2 - 10.1016/0166-0934(84)90040-5

DO - 10.1016/0166-0934(84)90040-5

M3 - Article

C2 - 6200490

AN - SCOPUS:0021354559

SN - 0166-0934

VL - 8

SP - 57

EP - 61

JO - Journal of Virological Methods

JF - Journal of Virological Methods

IS - 1-2

ER -

A method for increasing the surface details of resin embedded viruses using congo/crimean haemorrhagic fever virus as a model

Abstract

Bibliographical note

Keywords

Access to Document

Other files and links

Fingerprint

Cite this