A method for increasing the surface details of resin embedded viruses using congo/crimean haemorrhagic fever virus as a model

D. S. Ellis*, M. D. Smith, Graham Lloyd, E. T.W. Bowen, D. I.H. Simpson

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    Abstract

    The surface details of resin embedded viruses can be greatly enhanced by the use of uranyl acetate block staining. The virions are embedded in agar immediately after fixation and block stained with 3% uranyl acetate in methanol during dehydration. This technique allows those particles outside their host cells to show clearly the spikes normally seen only by the separate use of negative staining.

    Original languageEnglish
    Pages (from-to)57-61
    Number of pages5
    JournalJournal of Virological Methods
    Volume8
    Issue number1-2
    DOIs
    Publication statusPublished - Feb 1984

    Bibliographical note

    Funding Information:
    This work wass upportedb y a grantf rom theW ellcomeT rust. The authorsw isht o thank Springer-VerlagV, ienna, for permissiont o re-useF igs. 1, 2a nd 4 which had previouslya ppearedin Archiveso f Virology (Ellis et al., 1981).

    Keywords

    • electron microscopy staining
    • virus visualisation

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