A guinea pig model of low-dose Mycobacterium bovis aerogenic infection

Mark A. Chambers; Ann Williams; Dolores Gavier-Widén; Adam Whelan; Catherine Hughes; Graham Hall; M. Stephen Lever; Philip D. Marsh; R. Glyn Hewinson

doi:10.1016/S0378-1135(00)00378-3

A guinea pig model of low-dose Mycobacterium bovis aerogenic infection

Mark A. Chambers^*, Ann Williams, Dolores Gavier-Widén, Adam Whelan, Catherine Hughes, Graham Hall, M. Stephen Lever, Philip D. Marsh, R. Glyn Hewinson

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

30 Citations (Scopus)

Abstract

In order to develop a model of Mycobacterium bovis infection with pathogenetical relevance, a modified version of the Henderson apparatus was used to deliver infectious aerosols directly to the snouts of guinea pigs. Aerosols generated from 10⁶, 10⁷, 10⁸CFU/ml M. bovis suspensions established disease in every animal, with estimated retained doses of 10, 100, 1000CFU, respectively. For comparison, other guinea pigs were inoculated with 100CFU M. bovis intramuscularly (i.m.). Pathology and bacterial colonisation of lungs and spleen varied according to the dose and route of inoculation. Animals inoculated i.m. gave a significant cutaneous tuberculin hypersensitivity reaction earlier after testing than those infected aerogenically. A serological response to M. bovis antigens was detected in all infected animals. Intensity of antigen recognition was dose-dependent and although the range of antigens recognised varied between animals, a 25kDa antigen present in the cell fraction was serodominant. Thus, a reproducible guinea pig model has been defined that may be suitable for virulence, vaccination, and immunological studies.

Original language	English
Pages (from-to)	213-226
Number of pages	14
Journal	Veterinary Microbiology
Volume	80
Issue number	3
DOIs	https://doi.org/10.1016/S0378-1135(00)00378-3
Publication status	Published - 2001

Bibliographical note

Funding Information:
This work was supported by the Ministry of Agriculture, Fisheries, and Food, GB. We thank Wendy Pressling, VLA Weybridge, for the western blotting of guinea pig serum and the Animal Services Units at VLA Weybridge and CAMR. All animal work was undertaken in accordance with the requirements of the Home Office, UK and local Animal Care and Ethics Committees.

Keywords

Airborne infection
Guinea pig model
Mycobacterium bovis

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/S0378-1135(00)00378-3

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title = "A guinea pig model of low-dose Mycobacterium bovis aerogenic infection",

abstract = "In order to develop a model of Mycobacterium bovis infection with pathogenetical relevance, a modified version of the Henderson apparatus was used to deliver infectious aerosols directly to the snouts of guinea pigs. Aerosols generated from 106, 107, 108CFU/ml M. bovis suspensions established disease in every animal, with estimated retained doses of 10, 100, 1000CFU, respectively. For comparison, other guinea pigs were inoculated with 100CFU M. bovis intramuscularly (i.m.). Pathology and bacterial colonisation of lungs and spleen varied according to the dose and route of inoculation. Animals inoculated i.m. gave a significant cutaneous tuberculin hypersensitivity reaction earlier after testing than those infected aerogenically. A serological response to M. bovis antigens was detected in all infected animals. Intensity of antigen recognition was dose-dependent and although the range of antigens recognised varied between animals, a 25kDa antigen present in the cell fraction was serodominant. Thus, a reproducible guinea pig model has been defined that may be suitable for virulence, vaccination, and immunological studies.",

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note = "Funding Information: This work was supported by the Ministry of Agriculture, Fisheries, and Food, GB. We thank Wendy Pressling, VLA Weybridge, for the western blotting of guinea pig serum and the Animal Services Units at VLA Weybridge and CAMR. All animal work was undertaken in accordance with the requirements of the Home Office, UK and local Animal Care and Ethics Committees. ",

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AU - Williams, Ann

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AU - Hughes, Catherine

AU - Hall, Graham

AU - Lever, M. Stephen

AU - Marsh, Philip D.

AU - Hewinson, R. Glyn

N1 - Funding Information: This work was supported by the Ministry of Agriculture, Fisheries, and Food, GB. We thank Wendy Pressling, VLA Weybridge, for the western blotting of guinea pig serum and the Animal Services Units at VLA Weybridge and CAMR. All animal work was undertaken in accordance with the requirements of the Home Office, UK and local Animal Care and Ethics Committees.

PY - 2001

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N2 - In order to develop a model of Mycobacterium bovis infection with pathogenetical relevance, a modified version of the Henderson apparatus was used to deliver infectious aerosols directly to the snouts of guinea pigs. Aerosols generated from 106, 107, 108CFU/ml M. bovis suspensions established disease in every animal, with estimated retained doses of 10, 100, 1000CFU, respectively. For comparison, other guinea pigs were inoculated with 100CFU M. bovis intramuscularly (i.m.). Pathology and bacterial colonisation of lungs and spleen varied according to the dose and route of inoculation. Animals inoculated i.m. gave a significant cutaneous tuberculin hypersensitivity reaction earlier after testing than those infected aerogenically. A serological response to M. bovis antigens was detected in all infected animals. Intensity of antigen recognition was dose-dependent and although the range of antigens recognised varied between animals, a 25kDa antigen present in the cell fraction was serodominant. Thus, a reproducible guinea pig model has been defined that may be suitable for virulence, vaccination, and immunological studies.

AB - In order to develop a model of Mycobacterium bovis infection with pathogenetical relevance, a modified version of the Henderson apparatus was used to deliver infectious aerosols directly to the snouts of guinea pigs. Aerosols generated from 106, 107, 108CFU/ml M. bovis suspensions established disease in every animal, with estimated retained doses of 10, 100, 1000CFU, respectively. For comparison, other guinea pigs were inoculated with 100CFU M. bovis intramuscularly (i.m.). Pathology and bacterial colonisation of lungs and spleen varied according to the dose and route of inoculation. Animals inoculated i.m. gave a significant cutaneous tuberculin hypersensitivity reaction earlier after testing than those infected aerogenically. A serological response to M. bovis antigens was detected in all infected animals. Intensity of antigen recognition was dose-dependent and although the range of antigens recognised varied between animals, a 25kDa antigen present in the cell fraction was serodominant. Thus, a reproducible guinea pig model has been defined that may be suitable for virulence, vaccination, and immunological studies.

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A guinea pig model of low-dose Mycobacterium bovis aerogenic infection

Abstract

Bibliographical note

Keywords

UN SDGs

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