As whole-genome sequencing capacity becomes increasingly decentralized, there is a growing opportunity for collaboration and the sharing of surveillance data within and between countries to inform typhoid control policies. This vision requires free, community-driven tools that facilitate access to genomic data for public health on a global scale. Here we present the Pathogenwatch scheme for Salmonella enterica serovar Typhi (S. Typhi), a web application enabling the rapid identification of genomic markers of antimicrobial resistance (AMR) and contextualization with public genomic data. We show that the clustering of S. Typhi genomes in Pathogenwatch is comparable to established bioinformatics methods, and that genomic predictions of AMR are highly concordant with phenotypic susceptibility data. We demonstrate the public health utility of Pathogenwatch with examples selected from >4,300 public genomes available in the application. Pathogenwatch provides an intuitive entry point to monitor of the emergence and spread of S. Typhi high risk clones.
Bibliographical noteFunding Information: We are grateful to Flora Stevens and Joanne Freedman from the Travel Health and IHR department at Public Health England for providing some of the travel information linked to isolates from the United Kingdom, and to Dr. Koji Yahara, Dr. Makoto Ohnishi and Dr. Masatomo Morita for providing the travel information linked to isolates from Japan. Pathogenwatch is developed with support from Li Ka Shing Foundation (Big Data Institute, University of Oxford) and Wellcome (grant number 099202). S.A. and D.M.A. are supported by the National Institute for Health Research (UK) Global Health Research Unit on genomic Surveillance of AMR (16_136_111) and by the Centre for Genomic Pathogen Surveillance (http:// pathogensurveillance.net). Z.A.D. received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement TyphiNET No 845681. L.S.B. is funded by Plan GenT (CDEI-06/20-B), Conselleria de Sanitat Universal i Salut Pública, Generalitat Valenciana (Valencia, Spain).
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Publisher Copyright: © 2021, The Author(s).
Citation: Argimón, S., Yeats, C.A., Goater, R.J. et al. A global resource for genomic predictions of antimicrobial resistance and surveillance of Salmonella Typhi at pathogenwatch. Nat Commun 12, 2879 (2021).