A European multicenter study of immunoblotting in serodiagnosis of Lyme borreliosis

J. Robertson*, E. Guy, N. Andrews, B. Wilske, P. Anda, M. Granström, U. Hauser, Y. Moosmann, V. Sambri, J. Schellekens, G. Stanek, J. Gray

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

148 Citations (Scopus)

Abstract

A European multicenter study of immunoblotting for the serodiagnosis of Lyme borreliosis showed considerable variation results obtained from tests with a panel of 227 serum samples. Six laboratories used different immunoblot methods, and a wide range of bands was detected in all the assays. Multivariable logistic regression analysis of data from individual laboratories was used to determine the most discriminatory bands for reliable detection of antibodies to Borrelia burgdorferi sensu lato. These bands were used to construct individual interpretation rules for the immunoblots used in the six laboratories. Further analysis identified a subset of eight bands, which were important in all the laboratories, although with variations in significance. Possible European rules, all closely related, were formulated from these bands, although there was no single rule that gave high levels of sensitivity and specificity for all the laboratories. This is a reflection of the wide range of methodologies used, especially the use of different species and strains of B. burgdorferi sensu lato. The panel of European rules provides a framework for immunoblot interpretation which may be adapted in relation to the characteristics of Lyme borreliosis in local areas.

Original languageEnglish
Pages (from-to)2097-2102
Number of pages6
JournalJournal of Clinical Microbiology
Volume38
Issue number6
DOIs
Publication statusPublished - 2000

Bibliographical note

Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.

Fingerprint

Dive into the research topics of 'A European multicenter study of immunoblotting in serodiagnosis of Lyme borreliosis'. Together they form a unique fingerprint.

Cite this