A controlled study on the characterisation of bioaerosols emissions from compost

Zaheer Ahmad Nasir*, Catherine Rolph, Samuel Collins, David Stevenson, Toni L. Gladding, Enda Hayes, Ben Williams, Shagun Khera, Simon Jackson, Allan Bennett, Simon Parks, Robert P. Kinnersley, Kerry Walsh, Simon J.T. Pollard, Gillian Drew, Sonia Garcia Alcega, Frederic Coulon, Sean Tyrrel

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

23 Citations (Scopus)

Abstract

Bioaerosol emissions arising from biowaste treatment are an issue of public concern. To better characterise the bioaerosols, and to assess a range of measurement methods, we aerosolised green waste compost under controlled conditions. Viable and non-viable Andersen samplers, cyclone samplers and a real time bioaerosol detection system (Spectral Intensity Bioaerosol Sensor (SIBS)) were deployed simultaneously. The number-weighted fraction of fluorescent particles was in the range 22-26% of all particles for low and high emission scenarios. Overall fluorescence spectral profiles seen by the SIBS exhibited several peaks across the 16 wavelength bands from 298 to 735 nm. The size-fractionated endotoxin profile showed most endotoxin resided in the 2.1-9 μm aerodynamic diameter fraction, though up to 27% was found in a finer size fraction. A range of microorganisms were detected through culture, Matrix Assisted Laser Desorption and Ionisation Time of Flight Mass Spectrometry (MALDI-TOF) and quantitative polymerase chain reaction (qPCR), including Legionella pneumophila serogroup 1. These findings contribute to our knowledge of the physico-chemical and biological characteristics of bioaerosols from composting sites, as well as informing future monitoring approaches and data interpretation for bioaerosol measurement.

Original languageEnglish
Article number379
JournalAtmosphere
Volume9
Issue number10
DOIs
Publication statusPublished - 28 Sept 2018

Bibliographical note

Funding Information:
Funding: This research was funded by the Natural Environment Research Council (NE/M01163/1, NE/M011747/1, NE/M011763/1, NE/M011658/1 and NE/M010961/10). The APC was funded by (NE/M01163/1).

Funding Information:
This award is made under the auspices of the Environmental Microbiology and Human Health programme. This work represents the views of the authors, and not necessarily those of the funders and authors' associated institutions or organisations. The data will be available on 1 July 2020 via the Environmental Information Data Centre, the Natural Environment Research Council's long-term data repository for terrestrial and freshwater sciences https://catalogue.ceh.ac.uk/eidc/documents. This research was funded by the Natural Environment Research Council (NE/M01163/1, NE/M011747/1, NE/M011763/1, NE/M011658/1 and NE/M010961/10). The APC was funded by (NE/M01163/1).

Publisher Copyright:
© 2018 by the authors.

Keywords

  • Bioaerosols
  • Characterisation
  • Compost
  • Sampling methods

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