A comparison of two approaches to extracting Cryptosporidium DNA from human stools as measured by a real-time PCR assay

K. Elwin, G. Robinson, S. J. Hadfield, H. V. Fairclough, Miren Iturriza-Go'Mara, R. M. Chalmers*

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    28 Citations (Scopus)

    Abstract

    Direct extraction of Cryptosporidium DNA from 46 stools by bead-beating, guanidine thiocyanate and silica purification provided slightly lower PCR positivity (93.5% vs. 100%) and higher threshold cycle values (mean 34.93 vs. 28.03; P = 0.00) than spin-column extraction from boiled, semi-purified oocyst suspensions. However, direct extraction is cheaper, and amenable to automation.

    Original languageEnglish
    Pages (from-to)38-40
    Number of pages3
    JournalJournal of Microbiological Methods
    Volume89
    Issue number1
    DOIs
    Publication statusPublished - Apr 2012

    Bibliographical note

    Funding Information:
    We thank Jonathan Goss at the UK Cryptosporidium Reference Unit for performing the Method 1 DNA extractions. Hannah Fairclough was supported by a Students into Work grant from the Society for Applied Microbiology, who also part-funded this work.

    Keywords

    • Cryptosporidium
    • DNA extraction
    • Real time PCR

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